ArticleGenes & development2003
Physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment in S. pombe.
Article in Genes & development, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 62 citations in OpenAlex.
- A distinct phase of cyclin B (Cdc13) nuclear export at mitotic entry inOpen biology · 2025Article
- Article
- Characterization of temperature-sensitive alleles of anillin-like Mid1 and polo kinase Plo1 inmicroPublication biology · 2025Article
- CDK activity at the centrosome regulates the cell cycle.Cell reports · 2024Article
- An updated view on the centrosome as a cell cycle regulator.Cell division · 2022Review
- Redistribution of centrosomal proteins by centromeres and Polo kinase controls partial nuclear envelope breakdown in fission yeast.Molecular biology of the cell · 2021Article
- Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.Cell reports · 2018Article
- Dialogue between centrosomal entrance and exit scaffold pathways regulates mitotic commitment.The Journal of cell biology · 2017Article
- The centrosome and its duplication cycle.Cold Spring Harbor perspectives in biology · 2015Review
- Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast.Molecular biology of the cell · 2014Article
- Centrosomes as signalling centres.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body.Journal of cell science · 2014Article
- Cdk1 promotes cytokinesis in fission yeast through activation of the septation initiation network.Molecular biology of the cell · 2014Article
- Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis.Molecular biology of the cell · 2013Article
- Centrosomal MPF triggers the mitotic and morphogenetic switches of fission yeast.Nature cell biology · 2013Article
- Fission yeast Nod1 is a component of cortical nodes involved in cell size control and division site placement.PloS one · 2013Article
- Hsp90 interaction with Cdc2 and Plo1 kinases contributes to actomyosin ring condensation in fission yeast.Current genetics · 2012Article
- Spindle pole body components are reorganized during fission yeast meiosis.Molecular biology of the cell · 2012Article
- Spatiotemporal regulations of Wee1 at the G2/M transition.Molecular biology of the cell · 2011Article
- Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase.The EMBO journal · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Commitment to mitosis is regulated by a protein kinase complex called MPF. MPF is inhibited by Wee1-related kinases and activated by Cdc25 phosphatase. MPF activation further boosts Cdc25 and represses Wee1. This feedback control probably involves polo kinase. A dominant cut12.s11 mutation in the Schizosaccharomyces pombe spindle pole body (SPB) component Cut12 both suppresses the conditional lethal mitotic commitment defect of cdc25.22 and promotes premature association of the S. pombe polo kinase, Plo1, with the SPB. We now show that Cut12 associated with Plo1 in two hybrid and immunoprecipitation assays. Plo1 function was required for recognition of the mitotic SPB by the phospho-specific antibody MPM-2. In vivo MPM-2 staining and in vitro kinase assays established that the loss-of-function mutation, cut12.1, reduced mitotic activation of Plo1, whereas the gain-of-function mutation, cut12.s11, promoted higher levels of Plo1 activity than were normally seen in interphase. cut12.s11 could not promote mitotic commitment of cdc25.22 cells when Plo1 function was compromised. Expression of a constitutively active plo1 allele suppressed the mitotic commitment defect of cdc25.22. These data suggest that cut12.s11 suppresses cdc25.22 by promoting Plo1 activity. Furthermore, the delayed mitotic commitment of plo1.ts2 cells suggests that Plo1 is an integral part of the core controls that modulate MPF activation in S. pombe.
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