ArticleThe Biochemical journal1992
Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections.
Article in The Biochemical journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- The discovery of dystrophin, the protein product of the Duchenne muscular dystrophy gene.The FEBS journal · 2020Article
- Myoclonus dystonia and muscular dystrophy: ɛ-sarcoglycan is part of the dystrophin-associated protein complex in brain.Movement disorders : official journal of the Movement Disorder Society · 2016Article
- The ZZ domain of dystrophin in DMD: making sense of missense mutations.Human mutation · 2014Article
- Article
- Presynaptic calcium channels and α3-integrins are complexed with synaptic cleft laminins, cytoskeletal elements and active zone components.Journal of neurochemistry · 2010Article
- Reduction of high background staining by heating unfixed mouse skeletal muscle tissue sections allows for detection of thermostable antigens with murine monoclonal antibodies.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina.The Journal of comparative neurology · 2007Article
- Long-term persistence of donor nuclei in a Duchenne muscular dystrophy patient receiving bone marrow transplantation.The Journal of clinical investigation · 2002Article
- Different dystrophin-like complexes are expressed in neurons and glia.The Journal of cell biology · 1999Article
- Differential expression of dystrophin isoforms and utrophin during dibutyryl-cAMP-induced morphological differentiation of rat brain astrocytes.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- beta-dystrobrevin, a member of the dystrophin-related protein family.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- In situ molecular association of dystrophin with actin revealed by sensitized emission immuno-resonance energy transfer.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Specificity and VH sequence of two monoclonal antibodies against the N-terminus of dystrophin.The Biochemical journal · 1995Article
- Characterization of revertant muscle fibers in Duchenne muscular dystrophy, using exon-specific monoclonal antibodies against dystrophin.American journal of human genetics · 1995Article
- Monoclonal antibodies against the muscle-specific N-terminus of dystrophin: characterization of dystrophin in a muscular dystrophy patient with a frameshift deletion of exons 3-7.American journal of human genetics · 1993Article
- Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy.American journal of human genetics · 1993Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A group of 44 monoclonal antibodies (mAbs) raised against the central helical rod (25 mAbs) and C-terminal (19 mAbs) regions of dystrophin were prepared using trpE recombinant fusion proteins as immunogens. Some mAbs cross-react with the structurally related proteins, alpha-actinin and utrophin. Epitope mapping revealed uneven distribution of mAb-binding sites, no mAbs being produced against the C-terminal end of the helical fragment or the cysteine-rich region of the C-terminal dystrophin fragment. The failure of these large regions of the recombinant immunogens to elicit anti-dystrophin antibodies may be because of their inability to fold into the correct dystrophin-like conformation. The mAbs were selected for their ability to recognize 427 kDa dystrophin on Western blots after SDS/PAGE, and/or for immunostaining of the membrane in frozen muscle sections. Although some mAbs obtained by Western-blot screening failed to bind native dystrophin in frozen muscle sections, successful binding could be obtained after SDS or urea treatment of the tissue section to expose the epitopes. This increases the range of mAbs available for detection of dystrophin deletions in muscular dystrophy and evaluation of myoblast therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.