ArticleJournal of bacteriology2003
Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 247 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
247 citing papers in PubMed.
- Selection of fecal enterococci exhibiting tcrB-mediated copper resistance in pigs fed diets supplemented with copper.Applied and environmental microbiology · 2011Trial
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- Microbial functional traits in the hyperaccumulating Noccaea praecox rhizobiome are metal-dependent and host-driven.Environmental microbiome · 2026Article
- Global Analysis of the Co-Occurrence of Antimicrobial and Metal Resistance Genes in Bacterial Genomes From Dairy-Isolated Pathogens.Environmental microbiology · 2026Article
- Insights into the Performance of CusF as a Solubility Tag for Recombinant Protein Expression.International journal of molecular sciences · 2026Article
- Comparative genomic insights into the genus Pantoea: genetic determinants of ecological lifestyle diversity and plant growth-promoting potential.World journal of microbiology & biotechnology · 2026Article
- Dual regulation ofmicroLife · 2026Article
- Beyond antibiotic resistance: evidence for resistance-nodulation-division (RND) efflux pumps as virulence determinants.Microbiology and molecular biology reviews : MMBR · 2025Review
- Antimicrobial resistance is widespread among intestinal and extra-intestinalInfection and immunity · 2025Article
- The CueR-regulated transporters CopA and CusFABC coordinate copper detoxification inVirulence · 2025Article
- Genomic prediction of phytase potential and stress tolerance in maize-associated plant growth-promoting rhizobacterium Enterobacter cloacae Mz49.AMB Express · 2025Article
- A flow equilibrium model controlling cytoplasmic transition metal cation pools and preventing mis-metalation as exemplified for zinc homeostasis.Journal of bacteriology · 2025Review
- Role of the Multicopper Oxidase CueO in Copper Homeostasis Under Anaerobic Conditions in Enterobacteria.Molecular microbiology · 2025Article
- In Silico Design of a Multiepitope Vaccine Against Intestinal PathogenicDiseases (Basel, Switzerland) · 2025Article
- Biogenic Synthesis of Silver Nanoparticles and Their Diverse Biomedical Applications.Molecules (Basel, Switzerland) · 2025Review
- Review
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- A historical perspective on the multifunctional outer membrane channel protein TolC in Escherichia coli.npj antimicrobials and resistance · 2025Review
187 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The cus determinant of Escherichia coli encodes the CusCFBA proteins that mediate resistance to copper and silver by cation efflux. CusA and CusB were essential for copper resistance, and CusC and CusF were required for full resistance. Replacements of methionine residues 573, 623, and 672 with isoleucine in CusA resulted in loss of copper resistance, demonstrating their functional importance. Substitutions for several other methionine residues of this protein did not have any effect. The small 10-kDa protein CusF (previously YlcC) was shown to be a periplasmic protein. CusF bound one copper per polypeptide. The pink CusF copper protein complex exhibited an absorption maximum at around 510 nm. Methionine residues of CusF were involved in copper binding as shown by site-directed mutagenesis. CusF interacted with CusB and CusC polypeptides in a yeast two-hybrid assay. In contrast to other well-studied CBA-type heavy metal efflux systems, Cus was shown to be a tetrapartite resistance system that involves the novel periplasmic copper-binding protein CusF. These data provide additional evidence for the hypothesis that Cu(I) is directly transported from the periplasm across the outer membrane by the Cus complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.