ArticleThe Biochemical journal2003
Human salivary MUC7 mucin peptides: effect of size, charge and cysteine residues on antifungal activity.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Stage-Specific Mucin Reprogramming Across the Gastric Cancer Cascade: Unveiling Molecular Mechanisms and Novel Therapeutic Vulnerabilities.Biomolecules · 2026Review
- Modulating Copper(II) Coordination and Antimicrobial Activity: Effects of d-Amino Acid Substitution andInorganic chemistry · 2025Article
- Positive Role of Saliva in the Oral Microbiome.Advances in experimental medicine and biology · 2025Review
- Antimicrobial Peptides: Avant-Garde Antifungal Agents to Fight against Medically ImportantPharmaceutics · 2023Review
- View from the Biological Property: Insight into the Functional Diversity and Complexity of the Gut Mucus.International journal of molecular sciences · 2023Review
- The Potential Antimicrobial Action of Human Mucin 7 15-Mer Peptide and Its Metal Complexes.International journal of molecular sciences · 2021Article
- Characterization of a fungal competition factor: Production of a conidial cell-wall associated antifungal peptide.PLoS pathogens · 2020Article
- Mucus and Mucins: do they have a role in the inhibition of the human immunodeficiency virus?Virology journal · 2017Review
- Investigation of the N-terminal coding region ofBalkan journal of medical genetics : BJMG · 2016Article
- Hydrophobic mismatch demonstrated for membranolytic peptides, and their use as molecular rulers to measure bilayer thickness in native cells.Scientific reports · 2015Article
- Interplay between Candida albicans and the antimicrobial peptide armory.Eukaryotic cell · 2014Review
- The gastrointestinal mucus system in health and disease.Nature reviews. Gastroenterology & hepatology · 2013Review
- Chemical genomic screening of a Saccharomyces cerevisiae genomewide mutant collection reveals genes required for defense against four antimicrobial peptides derived from proteins found in human saliva.Antimicrobial agents and chemotherapy · 2013Article
- Influence of histatin 5 on Candida albicans mitochondrial protein expression assessed by quantitative mass spectrometry.Journal of proteome research · 2011Article
- Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts.Virology journal · 2010Article
- Antimicrobial peptide MUC7 12-mer activates the calcium/calcineurin pathway in Candida albicans.FEMS yeast research · 2010Article
- MUC7 polymorphisms are associated with a decreased risk of a diagnosis of asthma in an African American population.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2009Article
- Exploring the mode of action of antimicrobial peptide MUC7 12-mer by fitness profiling of Saccharomyces cerevisiae genomewide mutant collection.Antimicrobial agents and chemotherapy · 2009Article
- Factors affecting antimicrobial activity of MUC7 12-mer, a human salivary mucin-derived peptide.Annals of clinical microbiology and antimicrobials · 2007Article
- The ocular surface: the challenge to enable and protect vision: the Friedenwald lecture.Investigative ophthalmology & visual science · 2007Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have previously shown that MUC7 (human salivary low-molecular-mass mucin) 20-mer: LAHQKPFIRKSYKCLHKRCR (residues 32-51 of the parent MUC7, with a net positive charge of 7) possesses a broad-spectrum antimicrobial activity [Bobek and Situ (2003) Antimicrob. Agents Chemother. 47, 645-652]. The aims of the present study were to determine the minimum peptide chain length and its location within the 20-mer region that retains potent antifungal activity against Candida albicans and Cryptococcus neoformans and to examine the effect of net charge of the peptide as well as the role of cysteine residues on the fungicidal activity. First, several C-terminal truncated MUC7 20-mer fragments (16-mer, 12-mer, 11-mer, 10-mer and 8-mer) and one N-terminal fragment (8-mer-N) were synthesized and tested. The results showed that MUC7 12-mer, located at the C-terminal region of MUC7 20-mer, having a net charge of +6 and exhibiting an amphipathic helical conformation, not only retained but exceeded the antifungal activity of that of 20-mer. Secondly, several variants of the 12-mer peptide containing a lower or no net positive charge, or no cysteine residues were synthesized and tested. A clear correlation between the net positive charge of the 12-mer, its potency and initial interaction of peptide with fungal cells was found by killing assays, fluorescence microscopy and fungal cell-membrane potential measurements. Furthermore, cysteine residues, which play a critical role in bacterial binding, were found to be not important for the fungicidal activity of these peptides. These results identified MUC7 12-mer as a potential candidate for development into a novel antifungal therapeutic agent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.