ArticleThe Journal of experimental medicine1992
The contribution of increased collagen synthesis to human glomerulosclerosis: a quantitative analysis of alpha 2IV collagen mRNA expression by competitive polymerase chain reaction.
Article in The Journal of experimental medicine, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 71 citations in OpenAlex.
- SRY-related HMG box 9 regulates the expression of Col4a2 through transactivating its enhancer element in mesangial cells.The American journal of pathology · 2007Article
- Gene expression profiling analysis in nephrology: towards molecular definition of renal disease.Clinical and experimental nephrology · 2006Review
- The role of transglutaminase in the rat subtotal nephrectomy model of renal fibrosis.The Journal of clinical investigation · 1997Article
- EWS-Fli1 antisense oligodeoxynucleotide inhibits proliferation of human Ewing's sarcoma and primitive neuroectodermal tumor cells.The Journal of clinical investigation · 1997Article
- Extracellular matrix and the kidney.Journal of clinical pathology · 1996Review
- Use of semiquantitative PCR to assess onset and treatment of Pneumocystis carinii infection in rat model.Journal of clinical microbiology · 1995Article
- Constitutive expression and modulation of the functional thrombin receptor in the human kidney.The American journal of pathology · 1995Article
- Advanced glycation end products up-regulate gene expression found in diabetic glomerular disease.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Intraglomerular expression of transforming growth factor-beta 1 (TGF-beta 1) mRNA in patients with glomerulonephritis: quantitative analysis by competitive polymerase chain reaction.Clinical and experimental immunology · 1994Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
We previously reported that one of the main components of the sclerotic material in human glomerular diseases was type IV collagen. In this study we examined the contribution of increased synthesis to this process at the gene expression level. Sufficient material has not been available to study type IV collagen synthesis by normal or sclerotic glomeruli in humans. We took advantage of the availability of nephrectomy specimens from patients with renal carcinoma, and of the observation that approximately 50% of these patients develop varying degrees of glomerulosclerosis. We microdissected glomeruli from 10 patients and analyzed them using in situ reverse transcription coupled with polymerase chain reaction (PCR) analyses (in situ RT-PCR). alpha 2IV collagen mRNA, after reverse transcription into cDNA, was detected in all patients and appeared to be increased in those with glomerulosclerosis (n = 5). A competitive PCR assay was developed to quantitate this change. There was an average 3.7-fold increase in glomerular type IV collagen cDNA in patients with significant sclerosis. This change was not due to an increased number of glomerular cells. Thus, glomerulosclerosis in humans is associated with an elevation of glomerular type IV collagen gene expression, suggesting that increased synthesis of type IV collagen may represent one component of this process.
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