ArticleExtremophiles : life under extreme conditions2003
Purification and biological characterization of halocin C8, a novel peptide antibiotic from Halobacterium strain AS7092.
Article in Extremophiles : life under extreme conditions, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 69 citations in OpenAlex.
- Exploring the antimicrobial potential of two haloarchaeal strains belonging to the generaIranian journal of microbiology · 2025Article
- Halocins and CMarine drugs · 2024Review
- Halocin H4 is activated through cleavage by halolysin HlyR4.Applied and environmental microbiology · 2024Article
- Functional expression of an antimicrobial peptide, belonging to halocin C8 family, from Natrinema sp. RNS21 in Escherichia coli.Extremophiles : life under extreme conditions · 2023Article
- Comparative Genomics ofMicroorganisms · 2023Article
- Biotechnological potentials of halophilic microorganisms and their impact on mankind.Beni-Suef University journal of basic and applied sciences · 2022Review
- A Review on Microbial Products and Their Perspective Application as Antimicrobial Agents.Biomolecules · 2021Review
- Purification and Characterization of a New Halocin HA4 from Haloferax larsenii HA4 Isolated from a Salt Lake.Probiotics and antimicrobial proteins · 2021Article
- Halolysin R4 ofApplied and environmental microbiology · 2021Article
- Halophilic archaea and their potential to generate renewable fuels and chemicals.Biotechnology and bioengineering · 2021Review
- Antibiotics from Haloarchaea: What Can We Learn from Comparative Genomics?Marine biotechnology (New York, N.Y.) · 2020Article
- A Halocin Promotes DNA Uptake inFrontiers in microbiology · 2019Article
- Draft Genome Sequence ofGenome announcements · 2017Article
- Activity-guided separation and characterization of new halocin HA3 from fermented broth of Haloferax larsenii HA3.Extremophiles : life under extreme conditions · 2017Article
- Halocin C8: an antimicrobial peptide distributed among four halophilic archaeal genera: Natrinema, Haloterrigena, Haloferax, and Halobacterium.Extremophiles : life under extreme conditions · 2017Article
- Antagonistic interactions and production of halocin antimicrobial peptides among extremely halophilic prokaryotes isolated from the solar saltern of Sfax, Tunisia.Extremophiles : life under extreme conditions · 2016Article
- The identification of a novel Sulfolobus islandicus CAMP-like peptide points to archaeal microorganisms as cell factories for the production of antimicrobial molecules.Microbial cell factories · 2015Article
- Agar-supported cultivation of Halorubrum sp. SSR, and production of halocin C8 on the scale-up prototype Platotex.Extremophiles : life under extreme conditions · 2014Article
- Halocin SH10 production by an extreme haloarchaeon Natrinema sp. BTSH10 isolated from salt pans of South India.Saudi journal of biological sciences · 2013Article
- Interspecific Interactions Among Members of Family Halobacteriaceae from Natural Solar Salterns.Probiotics and antimicrobial proteins · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Halocins are bacteriocin-like proteins or peptides produced by many species of the family Halobacteriaceae. Halocin C8, excreted by the Halobacterium strain AS7092, is a single 6.3-kDa polypeptide with an isoelectric point of 4.4, which is sensitive to proteinase K but not to trypsin. Halocin C8 is quite stable, as it can be desalted, boiled, frozen, subjected to organic solvents, and stored in culture supernatant at 4 degrees C or in dH(2)O at -20 degrees C for more than 1 year without losing activity. The purification of this halocin was achieved by combination of tangential flow filtration (TFF), Sephadex G50 and DEAE-sepharose chromatography. The N-terminal amino acid sequence was also determined by Edman degradation. Halocin C8 appeared to have a very wide activity spectrum, including most haloarchaea and even some haloalkaliphilic rods. When a sensitive strain of Halorubrum saccharovorum was exposed to halocin C8, the treated cells swelled at the initial stage, the cell wall appeared to be nicked and the cytoplasm was then extruded out, and the whole cell was eventually completely lysed. These results indicate that halocin C8 is a novel microhalocin and its primary target might be located in the cell wall of the sensitive cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.