ArticleRNA (New York, N.Y.)2003
A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA.
Article in RNA (New York, N.Y.), 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 79 citations in OpenAlex.
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- Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites.Scientific reports · 2021Article
- SnoRNA copy regulation affects family size, genomic location and family abundance levels.BMC genomics · 2021Article
- Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs.Non-coding RNA · 2021Review
- Synergistic defects in pre-rRNA processing from mutations in the U3-specific protein Rrp9 and U3 snoRNA.Nucleic acids research · 2020Article
- Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis.RNA (New York, N.Y.) · 2019Article
- The role of RNA structure in translational regulation by L7Ae protein in archaea.RNA (New York, N.Y.) · 2019Article
- Epitranscriptomics of cancer.World journal of clinical oncology · 2018Review
- Implication of the box C/D snoRNP assembly factor Rsa1p in U3 snoRNP assembly.Nucleic acids research · 2017Article
- Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae.Protein science : a publication of the Protein Society · 2016Article
- The Kink Turn, a Key Architectural Element in RNA Structure.Journal of molecular biology · 2016Review
- Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex.BMC molecular biology · 2015Article
- The K-turn motif in riboswitches and other RNA species.Biochimica et biophysica acta · 2014Review
- Characterization of the interaction between protein Snu13p/15.5K and the Rsa1p/NUFIP factor and demonstration of its functional importance for snoRNP assembly.Nucleic acids research · 2014Article
- Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction.Nucleic acids research · 2014Article
- The molecular recognition of kink-turn structure by the L7Ae class of proteins.RNA (New York, N.Y.) · 2013Article
- Article
- A second base pair interaction between U3 small nucleolar RNA and the 5'-ETS region is required for early cleavage of the yeast pre-ribosomal RNA.Nucleic acids research · 2011Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 15.5-kD protein and its yeast homolog Snu13p bind U4 snRNA, U3 snoRNA, and the C/D box snoRNAs. In U4 snRNA, they associate with a helix-bulge-helix (K-turn) structure. U3 snoRNA contains two conserved pairs of boxes, C'/D and B/C, which were both expected to bind the 15.5-kD/Snu13 protein. Only binding to the B/C motif was experimentally demonstrated. Here, by chemical probing of in vitro reconstituted RNA/protein complexes, we demonstrate the independent binding of the 15.5-kD/Snu13 protein to each of the two motifs. Due to a highly reduced stem I (1 bp), the K-turn structure is not formed in the naked B/C motif. However, gel-shift experiments revealed a higher affinity of Snu13p for the B/C motif, compared to the C'/D motif. A phylogenetic analysis of U3 snoRNA, coupled with an analysis of Snu13p affinity for variant yeast C'/D and B/C motifs, and a study of the functionality of a truncated yeast U3 snoRNA carrying base substitutions in the C'/D and B/C motifs, revealed that conservation of the identities of residues 2 and 3 in the B/C K-turn is more important for Snu13p binding and U3 snoRNA function, than conservation of the identities of corresponding residues in the C'/D K-turn. This suggests that binding of Snu13p to K-turns with a very short helix I imposes sequence constraints in the bulge. Altogether, the data demonstrate the strong importance of the binding of the 15.5-kD/Snu13 protein to the C'/D and B/C motifs for both U3 snoRNP assembly and activity.
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