ArticleThe Journal of cell biology2003
ADP ribosylation factor 6 is activated and controls membrane delivery during phagocytosis in macrophages.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 191 citations in OpenAlex.
- Syntaxin-2 balances phagocytic uptake and phagolysosomal clearance in macrophages.Journal of cell science · 2025Article
- Phagocytosis at a glance.Journal of cell science · 2025Review
- Trophic and temporal dynamics of macrophage biology in human inner ear organogenesis.Frontiers in immunology · 2025Article
- Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages.Life science alliance · 2024Article
- Article
- Protrudin-mediated ER-endosome contact sites promote phagocytosis.Cellular and molecular life sciences : CMLS · 2023Article
- Membrane backtracking at the maximum capacity of nondigestible antigen phagocytosis in macrophages.Biophysical journal · 2023Article
- Review
- Large vesicle extrusions fromeLife · 2023Article
- Article
- A Novel Deoxynivalenol-Activated WheatJournal of fungi (Basel, Switzerland) · 2021Article
- FBP17-mediated finger-like membrane protrusions in cell competition between normal and RasV12-transformed cells.iScience · 2021Article
- Endocytosis and the internalization of pathogenic organisms: focus on phosphoinositides.F1000Research · 2020Review
- Membrane Dynamics and Organization of the Phagocyte NADPH Oxidase in PLB-985 Cells.Frontiers in cell and developmental biology · 2020Article
- Assessment of Arf6 Deletion in PLB-985 Differentiated in Neutrophil-Like Cells and in Mouse Neutrophils: Impact on Adhesion and Migration.Mediators of inflammation · 2020Article
- The C-terminal domain of EFA6A interacts directly with F-actin and assembles F-actin bundles.Scientific reports · 2019Article
- The role of MHC class I recycling and Arf6 in cross-presentation by murine dendritic cells.Life science alliance · 2019Article
- The myotubularin MTMR4 regulates phagosomal phosphatidylinositol 3-phosphate turnover and phagocytosis.The Journal of biological chemistry · 2019Article
- The Small GTPase Arf6: An Overview of Its Mechanisms of Action and of Its Role in Host⁻Pathogen Interactions and Innate Immunity.International journal of molecular sciences · 2019Review
- Arf GAPs as Regulators of the Actin Cytoskeleton-An Update.International journal of molecular sciences · 2019Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engulfment of particles by phagocytes is induced by their interaction with specific receptors on the cell surface, which leads to actin polymerization and the extension of membrane protrusions to form a closed phagosome. Membrane delivery from internal pools is considered to play an important role in pseudopod extension during phagocytosis. Here, we report that endogenous ADP ribosylation factor 6 (ARF6), a small GTP-binding protein, undergoes a sharp and transient activation in macrophages when phagocytosis was initiated via receptors for the Fc portion of immunoglobulins (FcRs). A dominant-negative mutant of ARF6 (T27N mutation) dramatically affected FcR-mediated phagocytosis. Expression of ARF6-T27N lead to a reduction in the focal delivery of vesicle-associated membrane protein 3+ endosomal recycling membranes at phagocytosis sites, whereas actin polymerization was unimpaired. This resulted in an early blockade in pseudopod extension and accumulation of intracellular vesicles, as observed by electron microscopy. We conclude that ARF6 is a major regulator of membrane recycling during phagocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.