ArticleThe Biochemical journal2003
Phosphorylation of BATF regulates DNA binding: a novel mechanism for AP-1 (activator protein-1) regulation.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- The transcription factor BATF pioneers the differentiation program of effector CD8Cell reports · 2025Article
- The role of BATF in immune cell differentiation and autoimmune diseases.Biomarker research · 2025Review
- Suppression of FOXP3 expression by the AP-1 family transcription factor BATF3 requires partnering with IRF4.Frontiers in immunology · 2022Article
- Strategies for Improving Photodynamic Therapy Through Pharmacological Modulation of the Immediate Early Stress Response.Methods in molecular biology (Clifton, N.J.) · 2022Review
- IFP35 Is a Relevant Factor in Innate Immunity, Multiple Sclerosis, and Other Chronic Inflammatory Diseases: A Review.Biology · 2021Review
- Phosphorylation within the bipartite NLS alters the localization and toxicity of the ER stress response factor DDIT3/CHOP.Cellular signalling · 2020Article
- OsbZIP42 is a positive regulator of ABA signaling and confers drought tolerance to rice.Planta · 2019Article
- Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop.Nature communications · 2016Article
- bZIPs and WRKYs: two large transcription factor families executing two different functional strategies.Frontiers in plant science · 2014Review
- Specificity through cooperation: BATF-IRF interactions control immune-regulatory networks.Nature reviews. Immunology · 2013Review
- Elucidation of functional markers from Aspergillus nidulans developmental regulator FlbB and their phylogenetic distribution.PloS one · 2011Article
- Batf promotes growth arrest and terminal differentiation of mouse myeloid leukemia cells.Molecular cancer research : MCR · 2011Article
- Transcriptional regulation of T helper 17 cell differentiation.Yonsei medical journal · 2010Review
- Identification of a novel DNA binding site and a transcriptional target for activating transcription factor 5 in c6 glioma and mcf-7 breast cancer cells.Molecular cancer research : MCR · 2009Article
- Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells.Annual review of biophysics · 2008Review
- Bimolecular fluorescence complementation: visualization of molecular interactions in living cells.Methods in cell biology · 2008Article
- Visualization of molecular interactions by fluorescence complementation.Nature reviews. Molecular cell biology · 2006Review
- Reduction/oxidation-phosphorylation control of DNA binding in the bZIP dimerization network.BMC genomics · 2006Article
- Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells.Nature protocols · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
BATF is a member of the AP-1 (activator protein-1) family of bZIP (basic leucine zipper) transcription factors that form transcriptionally inhibitory, DNA binding heterodimers with Jun proteins. In the present study, we demonstrate that BATF is phosphorylated in vivo on multiple serine and threonine residues and at least one tyrosine residue. Reverse-polarity PAGE revealed that serine-43 and threonine-48 within the DNA binding domain of BATF are phosphorylated. To model phosphorylation of the BATF DNA binding domain, serine-43 was replaced by an aspartate residue. BATF(S43D) retains the ability to dimerize with Jun proteins in vitro and in vivo, and the BATF(S43D):Jun heterodimer localizes properly to the nucleus of cells. Interestingly, BATF(S43D) functions like wild-type BATF to reduce AP-1-mediated gene transcription, despite the observed inability of the BATF(S43D):Jun heterodimer to bind DNA. These data demonstrate that phosphorylation of serine-43 converts BATF from a DNA binding into a non-DNA binding inhibitor of AP-1 activity. Given that 40% of mammalian bZIP transcription factors contain a residue analogous to serine-43 of BATF in their DNA binding domains, the phosphorylation event described here represents a mechanism that is potentially applicable to the regulation of many bZIP proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.