ArticleProceedings of the National Academy of Sciences of the United States of America2003
Small nucleolar RNA interference induced by antisense or double-stranded RNA in trypanosomatids.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- The Versatile Applications of Antisense Oligonucleotides in Modern Medicine.International journal of molecular sciences · 2026Review
- A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.Nature communications · 2023Article
- Visualizing nuclear RNAi activity in single living human cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Evolutionary Loss of RNAi Key Determinants in Kinetoplastids as a Multiple Sporadic Phenomenon.Journal of molecular evolution · 2017Article
- Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.Nature biotechnology · 2016Article
- Gene regulation of pteridine reductase 1 in leishmania promastigotes and amastigotes using a full-length antisense construct.Iranian journal of parasitology · 2013Article
- RNA-seq analysis of small RNPs in Trypanosoma brucei reveals a rich repertoire of non-coding RNAs.Nucleic acids research · 2012Article
- Efficient and specific knockdown of small non-coding RNAs in mammalian cells and in mice.Nucleic acids research · 2011Article
- Small nucleolar RNA interference in Trypanosoma brucei: mechanism and utilization for elucidating the function of snoRNAs.Nucleic acids research · 2010Article
- Methodological obstacles in knocking down small noncoding RNAs.RNA (New York, N.Y.) · 2009Article
- Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites.Nucleic acids research · 2009Article
- The subcellular distribution of an RNA quality control protein, the Ro autoantigen, is regulated by noncoding Y RNA binding.Molecular biology of the cell · 2009Article
- Psiscan: a computational approach to identify H/ACA-like and AGA-like non-coding RNA in trypanosomatid genomes.BMC bioinformatics · 2008Article
- A structured viroid RNA serves as a substrate for dicer-like cleavage to produce biologically active small RNAs but is resistant to RNA-induced silencing complex-mediated degradation.Journal of virology · 2007Article
- A genome-wide analysis of C/D and H/ACA-like small nucleolar RNAs in Trypanosoma brucei reveals a trypanosome-specific pattern of rRNA modification.RNA (New York, N.Y.) · 2005Article
- Effects of length and location on the cellular response to double-stranded RNA.Microbiology and molecular biology reviews : MMBR · 2004Review
- Sequence-specific inhibition of small RNA function.PLoS biology · 2004Article
- On the role of RNA silencing in the pathogenicity and evolution of viroids and viral satellites.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Decoding the riddle: the dawn of RNAi for the study of gene-gene and gene-environment interactions.Environmental health perspectives · 2004Article
- RNA interference and mRNA silencing, 2004: how far will they reach?Molecular biology of the cell · 2004Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In trypanosomes the C/D- and H/ACA-like small nucleolar RNAs (snoRNAs) are clustered and repeated in the genome. The snoRNAs studied to date are transcribed as polycistronic transcripts by RNA polymerase II and then processed, resulting in mature snoRNAs. In this study we demonstrated that snoRNA genes can be silenced in three trypanosomatid species: Leptomonas collosoma, Leishmania major, and Trypanosoma brucei. Silencing was achieved in L. collosoma and L. major by the expressing of an antisense transcript complementary to the snoRNA gene and was accompanied by the accumulation of small interfering RNA. Silencing eliminated the mature snoRNA but not its precursor and abolished the specific 2'-O-methylation guided by the snoRNA. In T. brucei, silencing was achieved by using the inducible synthesis of double-stranded RNA from T7 opposing promoters. Silencing varied between the different snoRNA genes, which may reflect the accessibility of small interfering RNA to the target RNAs. This study suggests that RNA interference can degrade snoRNAs. This study has further implications in elucidating the function of nucleolar RNAs and specific modifications guided by these RNAs in trypanosomatids and perhaps in other eukaryotes as well.
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