ArticleMolecular biology of the cell2003
The Chlamydomonas Fus1 protein is present on the mating type plus fusion organelle and required for a critical membrane adhesion event during fusion with minus gametes.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 95 citations in OpenAlex.
- GEX3 interacts with GEX2 to function in gamete attachment and plasma membrane fusion in Arabidopsis.Plant communications · 2026Article
- Molecular determinants of species-specific gamete adhesion triggering HAP2-mediated membrane fusion.Nature communications · 2026Article
- Sexual reproduction in land plants: an evolutionary perspective.Plant reproduction · 2025Review
- Cell size and selection for stress-induced cell fusion in unicellular eukaryotes.PLoS computational biology · 2025Article
- Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes.The Journal of cell biology · 2023Article
- Characteristics of NGenomics, proteomics & bioinformatics · 2023Article
- SPE-51, a sperm-secreted protein with an immunoglobulin-like domain, is required for fertilization in C. elegans.Current biology : CB · 2023Article
- Divergent molecular signatures in fish Bouncer proteins define cross-fertilization boundaries.Nature communications · 2023Article
- Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii.Communications biology · 2023Article
- Evolutionary Relationships and Divergence ofGenes · 2022Article
- DMP8 and 9 regulate HAP2/GCS1 trafficking for the timely acquisition of sperm fusion competence.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Uncovering an ancestral green ménage à trois: Contributions of Chlamydomonas to the discovery of a broadly conserved triad of plant fertilization proteins.Current opinion in plant biology · 2022Review
- Live imaging-based assay for visualising species-specific interactions in gamete adhesion molecules.Scientific reports · 2022Article
- Crossing and selection of Chlamydomonas reinhardtii strains for biotechnological glycolate production.Applied microbiology and biotechnology · 2022Article
- MAR1 links membrane adhesion to membrane merger during cell-cell fusion in Chlamydomonas.Developmental cell · 2021Article
- Species-specific gamete recognition initiates fusion-driving trimer formation by conserved fusogen HAP2.Nature communications · 2021Article
- Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma in Mice and Rats.Frontiers in cell and developmental biology · 2021Article
- Fusexins, HAP2/GCS1 and Evolution of Gamete Fusion.Frontiers in cell and developmental biology · 2021Review
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- New advances and future directions in plant polyspermy.Molecular reproduction and development · 2020Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The molecular mechanisms of the defining event in fertilization, gamete fusion, remain poorly understood. The FUS1 gene in the unicellular, biflagellated green alga Chlamydomonas is one of the few sex-specific eukaryotic genes shown by genetic analysis to be essential for gamete fusion during fertilization. In Chlamydomonas, adhesion and fusion of the plasma membranes of activated mt+ and mt- gametes is accomplished via specialized fusion organelles called mating structures. Herein, we identify the endogenous Fus1 protein, test the idea that Fus1 is at the site of fusion, and identify the step in fusion that requires Fus1. Our results show that Fus1 is a approximately 95-kDa protein present on the external surface of both unactivated and activated mt+ gametes. Bioassays indicate that adhesion between mating type plus and mating type minus fusion organelles requires Fus1 and that Fus1 is functional only after gamete activation. Finally, immunofluorescence demonstrates that the Fus1 protein is present as an apical patch on unactivated gametes and redistributes during gamete activation over the entire surface of the microvillous-like activated plus mating structure, the fertilization tubule. Thus, Fus1 is present on mt+ gametes at the site of cell-cell fusion and essential for an early step in the fusion process.
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