ArticleMolecular biology of the cell2003
Tomographic evidence for continuous turnover of Golgi cisternae in Pichia pastoris.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 142 citations in OpenAlex.
- Close-up of vesicular ER exit sites by volume electron imaging using FIB-SEM.The Journal of cell biology · 2026Article
- Rethinking the Yeast Endomembrane System.Sub-cellular biochemistry · 2026Review
- The Golgi checkpoint: Golgi unlinking during G2 is necessary for spindle formation and cytokinesis.Life science alliance · 2024Article
- Reevaluating Golgi fragmentation and its implications in wound repair.Cell regeneration (London, England) · 2024Review
- The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella phaffii.Biotechnology for biofuels and bioproducts · 2022Article
- Design of a novel switchable antibody display system in Pichia pastoris.Applied microbiology and biotechnology · 2022Article
- Article
- COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers.The Journal of cell biology · 2021Article
- Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins.Applied microbiology and biotechnology · 2021Review
- ER arrival sites associate with ER exit sites to create bidirectional transport portals.The Journal of cell biology · 2020Article
- Organization of Xylan Production in the Golgi During Secondary Cell Wall Biosynthesis.Plant physiology · 2019Article
- A Kinetic View of Membrane Traffic Pathways Can Transcend the Classical View of Golgi Compartments.Frontiers in cell and developmental biology · 2019Review
- Stochastic Model of Maturation and Vesicular Exchange in Cellular Organelles.Biophysical journal · 2018Article
- Conserved Oligomeric Golgi and Neuronal Vesicular Trafficking.Handbook of experimental pharmacology · 2018Review
- Quantifying Golgi structure using EM: combining volume-SEM and stereology for higher throughput.Histochemistry and cell biology · 2017Review
- Regulation of ER-Golgi Transport Dynamics by GTPases in Budding Yeast.Frontiers in cell and developmental biology · 2017Review
- Is Wortmannin-Induced Reorganization of the trans-Golgi Network the Key to Explain Charasome Formation?Frontiers in plant science · 2016Article
- In situ structural analysis of Golgi intracisternal protein arrays.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Vesicles versus Tubes: Is Endoplasmic Reticulum-Golgi Transport in Plants Fundamentally Different from Other Eukaryotes?Plant physiology · 2015Review
- Mechanisms and Regulation of the Mitotic Inheritance of the Golgi Complex.Frontiers in cell and developmental biology · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic reticulum (tER) sites, making this organism ideal for structure-function studies of the secretory pathway. Here, we have used P. pastoris to test various models for Golgi trafficking. The experimental approach was to analyze P. pastoris tER-Golgi units by using cryofixed and freeze-substituted cells for electron microscope tomography, immunoelectron microscopy, and serial thin section analysis of entire cells. We find that tER sites and the adjacent Golgi stacks are enclosed in a ribosome-excluding "matrix." Each stack contains three to four cisternae, which can be classified as cis, medial, trans, or trans-Golgi network (TGN). No membrane continuities between compartments were detected. This work provides three major new insights. First, two types of transport vesicles accumulate at the tER-Golgi interface. Morphological analysis indicates that the center of the tER-Golgi interface contains COPII vesicles, whereas the periphery contains COPI vesicles. Second, fenestrae are absent from cis cisternae, but are present in medial through TGN cisternae. The number and distribution of the fenestrae suggest that they form at the edges of the medial cisternae and then migrate inward. Third, intact TGN cisternae apparently peel off from the Golgi stacks and persist for some time in the cytosol, and these "free-floating" TGN cisternae produce clathrin-coated vesicles. These observations are most readily explained by assuming that Golgi cisternae form at the cis face of the stack, progressively mature, and ultimately dissociate from the trans face of the stack.
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