ArticleMolecular biology of the cell2003
She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 69 citations in OpenAlex.
- Yeast Deletomics to Uncover Gadolinium Toxicity Targets and Resistance Mechanisms.Microorganisms · 2023Article
- UCS Chaperone Folding of the Myosin Head: A Function That Evolved before Animals and Fungi Diverged from a Common Ancestor More than a Billion Years Ago.Biomolecules · 2022Review
- Class I myosin mediated endocytosis and polarization growth is essential for pathogenicity of Magnaporthe oryzae.Applied microbiology and biotechnology · 2021Article
- Phospholipid flippases and Sfk1 are essential for the retention of ergosterol in the plasma membrane.Molecular biology of the cell · 2021Article
- Pathogenic Variants in the Myosin Chaperone UNC-45B Cause Progressive Myopathy with Eccentric Cores.American journal of human genetics · 2020Article
- Unbiased yeast screens identify cellular pathways affected in Niemann-Pick disease type C.Life science alliance · 2020Article
- UCS protein function is partially restored in the Saccharomyces cerevisiae she4 mutant with expression of the human UNC45-GC, but not UNC45-SM.Cell stress & chaperones · 2018Article
- UNC-45a promotes myosin folding and stress fiber assembly.The Journal of cell biology · 2017Article
- Mutation of the Ser18 phosphorylation site on the sole Saccharomyces cerevisiae UCS protein, She4, can compromise high-temperature survival.Cell stress & chaperones · 2017Article
- A quantitative imaging-based screen reveals the exocyst as a network hub connecting endocytosis and exocytosis.Molecular biology of the cell · 2015Article
- Getting folded: chaperone proteins in muscle development, maintenance and disease.Anatomical record (Hoboken, N.J. : 2007) · 2014Review
- Myosin chaperones.Current opinion in structural biology · 2014Review
- Of social molecules: The interactive assembly of ASH1 mRNA-transport complexes in yeast.RNA biology · 2014Review
- Here, there, everywhere. mRNA localization in budding yeast.RNA biology · 2014Review
- The UNC-45 myosin chaperone: from worms to flies to vertebrates.International review of cell and molecular biology · 2014Review
- Article
- UCS protein Rng3p is essential for myosin-II motor activity during cytokinesis in fission yeast.PloS one · 2013Article
- Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast.The Journal of cell biology · 2012Article
- Dual function of the UNC-45b chaperone with myosin and GATA4 in cardiac development.Journal of cell science · 2012Article
- Principles of mRNA transport in yeast.Cellular and molecular life sciences : CMLS · 2012Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
She4p/Dim1p, a member of the UNC-45/CRO1/She4p (UCS) domain-containing protein family, is required for endocytosis, polarization of actin cytoskeleton, and polarization of ASH1 mRNA in Saccharomyces cerevisiae. We show herein that She4p/Dim1p is involved in endocytosis and actin polarization through interactions with the type I myosins Myo3p and Myo5p. Two-hybrid and biochemical experiments showed that She4p/Dim1p interacts with the motor domain of Myo3/5p through its UCS domain. She4p/Dim1p was required for Myo5p localization to cortical patch-like structures. Using random mutagenesis of the motor region of MYO5, we identified four independent dominant point mutations that suppress the temperature-sensitive growth phenotype of the she4/dim1 null mutant. All of the amino acid substitutions caused by these mutations, V164I, N168I, N209S, and K377M, could suppress the defects of endocytosis and actin polarization of the she4/dim1 mutant as well. She4p/Dim1p also showed two-hybrid interactions with the motor domain of a type II myosin Myo1p and type V myosins Myo2p and Myo4p, and was required for proper localization of Myo4p, which regulates polarization of ASH1 mRNA. Our results suggest that She4p/Dim1p is required for structural integrity or regulation of the motor domain of unconventional myosins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.