ArticleThe Journal of physiology2003
T- and L-type Ca2+ currents in freshly dispersed smooth muscle cells from the human proximal urethra.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Cells and ionic conductances contributing to spontaneous activity in bladder and urethral smooth muscle.The Journal of physiology · 2026Review
- Orai storeoperated CaChannels (Austin, Tex.) · 2025Review
- CACNA1D overexpression and voltage-gated calcium channels in prostate cancer during androgen deprivation.Scientific reports · 2023Article
- The intracellular CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- CaThe Journal of physiology · 2018Article
- Effects of new-generation TMEM16A inhibitors on calcium-activated chloride currents in rabbit urethral interstitial cells of Cajal.Pflugers Archiv : European journal of physiology · 2017Article
- Modelling Human Colonic Smooth Muscle Cell Electrophysiology.Cellular and molecular bioengineering · 2017Article
- Article
- Interstitial cells: regulators of smooth muscle function.Physiological reviews · 2014Review
- T-type Ca2+ channels and the urinary and male genital tracts.Pflugers Archiv : European journal of physiology · 2014Review
- Ion channels of the mammalian urethra.Channels (Austin, Tex.) · 2014Review
- Functional characterization of voltage-dependent Ca(2+) channels in mouse pulmonary arterial smooth muscle cells: divergent effect of ROS.American journal of physiology. Cell physiology · 2013Article
- Electrical slow waves in the mouse oviduct are dependent on extracellular and intracellular calcium sources.American journal of physiology. Cell physiology · 2011Article
- Contribution of Kv2.1 channels to the delayed rectifier current in freshly dispersed smooth muscle cells from rabbit urethra.American journal of physiology. Cell physiology · 2011Article
- Muscular effects of orexin A on the mouse duodenum: mechanical and electrophysiological studies.The Journal of physiology · 2011Article
- Preservation of serotonin-mediated contractility in adult sheep pulmonary arteries following long-term high-altitude hypoxia.High altitude medicine & biology · 2011Article
- Inhibitory actions of mibefradil on steroidogenesis in mouse Leydig cells: involvement of Ca(2+) entry via the T-type Ca(2+) channel.Asian journal of andrology · 2010Article
- Voltage-dependent calcium channels of dog basilar artery.The Journal of physiology · 2007Article
- Spontaneous activity of lower urinary tract smooth muscles: correlation between ion channels and tissue function.The Journal of physiology · 2006Review
- A Ni(2+)-sensitive component of the ERG b-wave from the isolated bovine retina is related to E-type voltage-gated Ca(2+) channels.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The purpose of the present study was to characterise Ca2+ currents in smooth muscle cells isolated from biopsy samples taken from the proximal urethra of patients undergoing surgery for bladder or prostate cancer. Cells were studied at 37 degrees C using the amphotericin B perforated-patch configuration of the patch-clamp technique. Currents were recorded using Cs+-rich pipette solutions to block K+ currents. Two components of current, with electrophysiological and pharmacological properties typical of T- and L-type Ca2+ currents, were present in these cells. When steady-state inactivation curves for the L current were fitted with a Boltzmann equation, this yielded a V1/2 of -45+/-5 mV. In contrast, the T current inactivated with a V1/2 of -80+/-3 mV. The L currents were reduced in a concentration-dependent manner by nifedipine (ED50=159+/-54 nM) and Ni2+ (ED50=65+/-16 microM) but were enhanced when external Ca2+ was substituted with Ba2+. The T current was little affected by TTX, reduction in external Na+, application of nifedipine at concentrations below 300 nM or substitution of external Ca2+ with Ba2+, but was reduced by Ni2+ with an ED50 of 6+/-1 microM. When cells were stepped from -100 to -30 mV in Ca2+-free conditions, small inward currents could be detected. These were enhanced 40-fold in divalent-cation-free solution and blocked in a concentration-dependent manner by Mg2+ with an ED50 of 32+/-16 microM. These data support the idea that human urethral myocytes possess currents with electrophysiological and pharmacological properties typical of T- and L-type Ca2+ currents.
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Registered trials
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