ArticleGenetics2003
Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 189 papers.
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Who cites it
189 citing papers in PubMed.
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Dissecting adult plant resistance to stripe and leaf rust in advanced Indian wheat breeding lines through a genome-wide association study.BMC plant biology · 2026Article
- Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping.Nature plants · 2025Article
- Identification of Leaf Rust-Related Gene Signature in Wheat (Triticum Aestivum L.) Using High-Throughput Sequencing, Network Analysis, and Machine Learning Algorithms.Rice (New York, N.Y.) · 2025Article
- Genetic dissection of leaf rust resistance in a diversity panel of tetraploid wheat (Triticum turgidum).BMC plant biology · 2025Article
- Identification of leaf rust resistance loci in hard winter wheat using genome-wide association mapping.The plant genome · 2025Article
- A single NLR gene confers resistance to leaf and stripe rust in wheat.Nature communications · 2024Article
- Puccinia triticina avirulence protein AvrLr21 directly interacts with wheat resistance protein Lr21 to activate wheat immune response.Communications biology · 2024Article
- Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Fine-mapping of LrN3B on wheat chromosome arm 3BS, one of the two complementary genes for adult-plant leaf rust resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Genome-wide atlas of rust resistance loci in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Genome wide association study of seedling and adult plant leaf rust resistance in two subsets of barley genetic resources.Scientific reports · 2024Article
- Phenotyping and Exploitation of Kompetitive Allele-Specific PCR Assays for Genes Underpinning Leaf Rust Resistance in New Spring Wheat Mutant Lines.Current issues in molecular biology · 2024Article
- Article
- The Underexplored Mechanisms of Wheat Resistance to Leaf Rust.Plants (Basel, Switzerland) · 2023Review
- Identification and introgression of a novel leaf rust resistance gene from Thinopyrum intermedium chromosome 7JTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Genetic mapping of the wheat leaf rust resistance gene Lr19 and development of translocation lines to break its linkage with yellow pigment.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Identification of the Solid Stem Suppressor GeneInternational journal of molecular sciences · 2023Article
- Integrated Molecular and Bioinformatics Approaches for Disease-Related Genes in Plants.Plants (Basel, Switzerland) · 2023Review
- Comparative transcriptome profiling of near isogenic lines PBW343 and FLW29 to unravel defense related genes and pathways contributing to stripe rust resistance in wheat.Functional & integrative genomics · 2023Article
129 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a gene-rich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.
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