ArticleMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada2003
Imaging mitochondrial organization in living primate oocytes and embryos using multiphoton microscopy.
Article in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 75 citations in OpenAlex.
- The Istanbul consensus update: a revised ESHRE/ALPHA consensus on oocyte and embryo static and dynamic morphological assessment†,‡.Human reproduction (Oxford, England) · 2025Article
- Review
- Mitochondria Lead the Way: Mitochondrial Dynamics and Function in Cellular Movements in Development and Disease.Frontiers in cell and developmental biology · 2021Review
- Raman spectroscopy on live mouse early embryo while it continues to develop into blastocyst in vitro.Scientific reports · 2019Article
- Mitochondria in Embryogenesis: An Organellogenesis Perspective.Frontiers in cell and developmental biology · 2019Article
- Pluripotent Stem Cell Metabolism and Mitochondria: Beyond ATP.Stem cells international · 2017Review
- Metabolic restructuring and cell fate conversion.Cellular and molecular life sciences : CMLS · 2015Review
- Pluripotent stem cell energy metabolism: an update.The EMBO journal · 2015Review
- The relationship between pluripotency and mitochondrial DNA proliferation during early embryo development and embryonic stem cell differentiation.Stem cell reviews and reports · 2009Review
- Optical spectroscopy and imaging for the noninvasive evaluation of engineered tissues.Tissue engineering. Part B, Reviews · 2008Review
- Mitochondria in stem cells.Mitochondrion · 2007Review
- Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: implications for developmental potential.Developmental biology · 2006Article
- Comparative maturation of cynomolgus monkey oocytes in vivo and in vitro.Reproductive biology and endocrinology : RB&E · 2006Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We employed multiphoton laser scanning microscopy (MPLSM) to image changes in mitochondrial distribution in living rhesus monkey embryos. This method of imaging does not impair development; thus, the same specimen can be visualized multiple times at various developmental stages. Not only does this increase the amount of information that can be gathered on a single specimen but it permits the correlation of early events with subsequent development in the same specimen. Here we demonstrate the utility of MPLSM for determining changes in mitochondrial organization at various developmental stages and show that rhesus zygotes possess a distinct accumulation of mitochondria between the pronuclei prior to syngamy. We present evidence that suggests that this pronuclear accumulation may be positively correlated with development to the blastocyst stage-in the same embryo-thereby illustrating how MPLSM can be used to correlate cellular dynamics of primate oocytes and early embryos with their developmental potential. Understanding the relationship between mitochondrial distribution and the subsequent development of mammalian embryos, particularly primates, will increase our ability to improve embryo culture technologies, including those used for human assisted reproduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.