ArticlePlant physiology2003
A resource of mapped dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 27 citations in OpenAlex.
- The rice miniature inverted repeat transposable element mPing is an effective insertional mutagen in soybean.Plant physiology · 2011Article
- A candidate gene OsAPC6 of anaphase-promoting complex of rice identified through T-DNA insertion.Functional & integrative genomics · 2010Article
- Targeted Ds-tagging strategy generates high allelic diversity at the Arabidopsis HY2 locus.Plant molecular biology · 2006Article
- Dissociation (Ds) constructs, mapped Ds launch pads and a transiently-expressed transposase system suitable for localized insertional mutagenesis in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Article
- Distribution of Activator (Ac) throughout the maize genome for use in regional mutagenesis.Genetics · 2005Article
- An inducible targeted tagging system for localized saturation mutagenesis in Arabidopsis.Plant physiology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a new resource for targeted insertional mutagenesis in Arabidopsis using a maize (Zea mays) Activator/Dissociation (Ds) two-element system. The two components of the system, T-DNA vectors carrying a Ds launch pad and a stable Activator transposase source, were designed to simplify selection of transposition events and maximize their usefulness. Because Ds elements preferentially transpose to nearby genomic sites, they can be used in targeted mutagenesis of linked genes. To efficiently target all genes throughout the genome, we generated a large population of transgenic Arabidopsis plants containing the Ds launch pad construct, identified lines containing single Ds launch pad inserts, and mapped the positions of Ds launch pads in 89 lines. The integration sites of the Ds launch pads were relatively evenly distributed on all five chromosomes, except for a region of chromosomes 2 and 4 and the centromeric regions. This resource therefore provides access to the majority of the Arabidopsis genome for targeted tagging.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.