ArticleProceedings of the National Academy of Sciences of the United States of America2003
The coiled-coil of the human Rad50 DNA repair protein contains specific segments of increased flexibility.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 88 citations in OpenAlex.
- Article
- DNA Damage and Replication Stress Checkpoints.Annual review of biochemistry · 2025Review
- Dynamic Properties of the DNA Damage Response Mre11/Rad50 Complex.International journal of molecular sciences · 2023Review
- Article
- Three segment ligation of a 104 kDa multi-domain protein by SrtA and OaAEP1.Journal of biomolecular NMR · 2023Article
- Measurement of the persistence length of cytoskeletal filaments using curvature distributions.Biophysical journal · 2022Article
- The dynamic nature of the Mre11-Rad50 DNA break repair complex.Progress in biophysics and molecular biology · 2021Review
- A Disease-Causing Single Amino Acid Deletion in the Coiled-Coil Domain of RAD50 Impairs MRE11 Complex Functions in Yeast and Humans.Cell reports · 2020Article
- Hitching a Ride: Mechanics of Transport Initiation through Linker-Mediated Hitchhiking.Biophysical journal · 2020Article
- Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge.Nature communications · 2020Article
- Functional and structural insights into the MRX/MRN complex, a key player in recognition and repair of DNA double-strand breaks.Computational and structural biotechnology journal · 2020Review
- Sharp kinking of a coiled-coil in MutS allows DNA binding and release.Nucleic acids research · 2019Article
- Modular assembly of a protein nanotriangle using orthogonally interacting coiled coils.Scientific reports · 2017Article
- Eukaryotic resectosomes: A single-molecule perspective.Progress in biophysics and molecular biology · 2017Review
- Architectural plasticity of human BRCA2-RAD51 complexes in DNA break repair.Nucleic acids research · 2017Article
- DNA End Resection: Facts and Mechanisms.Genomics, proteomics & bioinformatics · 2016Review
- Condensin Smc2-Smc4 Dimers Are Flexible and Dynamic.Cell reports · 2016Article
- Envisioning the dynamics and flexibility of Mre11-Rad50-Nbs1 complex to decipher its roles in DNA replication and repair.Progress in biophysics and molecular biology · 2015Review
- ATPase-dependent control of the Mms21 SUMO ligase during DNA repair.PLoS biology · 2015Article
- Three-dimensional topology of the SMC2/SMC4 subcomplex from chicken condensin I revealed by cross-linking and molecular modelling.Open biology · 2015Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein structural features are usually determined by defining regularities in a large population of homogeneous molecules. However, irregular features such as structural variation and flexibility are likely to be missed, despite their vital role for their biological function. In this paper, we report the observation of striking irregularities in the flexibility of the coiled-coil region of the human Rad50 DNA repair protein. Existing methods to quantitatively analyze flexibility are applicable to homogeneous polymers only. Because protein coiled-coils cannot be assumed to be homogeneous, we develop a method to quantify the local flexibility from high-resolution atomic force microscopy images. Indeed, in Rad50 coiled-coils, two positions of increased flexibility are observed. We discuss how this dynamic structural feature is integral to Rad50 function.
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