ArticleJournal of virology2003
Functional analysis of the endogenous retroviral promoter of the human endothelin B receptor gene.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 64 citations in OpenAlex.
- Species-Specific Transcription Factors Associated with Long Terminal Repeat Promoters of Endogenous Retroviruses: A Comprehensive Review.Biomolecules · 2024Review
- Characterization of NFE2L1-616, an isoform of nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes.Scientific reports · 2023Article
- Anthropoid primate-specific retroviral element THE1B controls expression of CRH in placenta and alters gestation length.PLoS biology · 2018Article
- A survey of endogenous retrovirus (ERV) sequences in the vicinity of multiple sclerosis (MS)-associated single nucleotide polymorphisms (SNPs).Molecular biology reports · 2016Article
- Evolutionary impact of transposable elements on genomic diversity and lineage-specific innovation in vertebrates.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Review
- Association of endogenous retroviruses and long terminal repeats with human disorders.Frontiers in oncology · 2013Review
- Is there a role for endogenous retroviruses to mediate long-term adaptive phenotypic response upon environmental inputs?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Early progress in epigenetic regulation of endothelin pathway genes.British journal of pharmacology · 2013Review
- Genomic impact, chromosomal distribution and transcriptional regulation of HERV elements.Molecules and cells · 2012Review
- Hypomethylation of functional retrotransposon-derived genes in the human placenta.Mammalian genome : official journal of the International Mammalian Genome Society · 2011Article
- Placenta-specific expression of the interleukin-2 (IL-2) receptor β subunit from an endogenous retroviral promoter.The Journal of biological chemistry · 2011Article
- Establishing the baseline level of repetitive element expression in the human cortex.BMC genomics · 2011Article
- Article
- Widely variable endogenous retroviral methylation levels in human placenta.Nucleic acids research · 2007Article
- At least 50% of human-specific HERV-K (HML-2) long terminal repeats serve in vivo as active promoters for host nonrepetitive DNA transcription.Journal of virology · 2006Article
- Prolactin in man: a tale of two promoters.BioEssays : news and reviews in molecular, cellular and developmental biology · 2006Review
- Molecular evolution of the periphilin gene in relation to human endogenous retrovirus m element.Journal of molecular evolution · 2006Article
- Expression profiles of endogenous retroviruses in Old World monkeys.Journal of virology · 2006Article
- Recently mobilized transposons in the human and chimpanzee genomes.American journal of human genetics · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported that the long terminal repeats (LTRs) of retroviral elements belonging to the HERV-E family contribute to the expression of the human apolipoprotein C1 (APOC1) and endothelin B receptor (EDNRB) genes by providing alternative promoters. While both LTRs were shown to promote transcription in vivo and in vitro, their respective activity and tissue specificity appeared to differ even though they shared a high degree of sequence identity. In the present study, we further characterized the promoter of the EDNRB LTR and delineated the regions and motifs required for strong activity. We confirmed the placenta-restricted expression of the LTR by transient transfections and quantitative real-time PCR and determined that the retroviral promoter contributes significantly to the level of EDNRB transcripts in placenta, where chimeric mRNAs were found to represent 15% of overall EDNRB mRNAs. Transient transfection of 5' deletion constructs in cells of placental origin identified a motif, named LPE1, between positions 111 and 122 of the EDNRB LTR necessary for transcriptional activity. Removal of this region, which contains a putative SP1 binding site, abolished promoter activity. A second enhancing region resides between positions 175 and 215 of the LTR and was termed LPE2. Interestingly, this section contained three binding sites that were not present in the APOC1 LTR due to minor nucleotide differences. The predicted motifs in the EDNRB LTR were found to likely act in symbiosis as modifications to any of the three sites reduced transcription by one-third while alterations to all three eliminated promoter activity. The results from this study illustrate how slight variations in transcriptional regulatory sequences can have a profound effect on promoter activity and demonstrate the complex regulatory effects of human endogenous retrovirus elements on human gene expression.
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