ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
The distribution of calcium buffering proteins in the turtle cochlea.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- Avian auditory hair cell regeneration is accompanied by JAK/STAT-dependent expression of immune-related genes in supporting cells.Development (Cambridge, England) · 2022Article
- Calretinin Immunoreactivity in the VIIIth Nerve and Inner Ear Endorgans ofFrontiers in neuroscience · 2021Article
- Serial scanning electron microscopy of anti-PKHD1L1 immuno-gold labeled mouse hair cell stereocilia bundles.Scientific data · 2020Article
- Tonotopy in calcium homeostasis and vulnerability of cochlear hair cells.Hearing research · 2019Review
- Differential Gene Expression Patterns Between Apical and Basal Inner Hair Cells Revealed by RNA-Seq.Frontiers in molecular neuroscience · 2019Article
- A Model for Link Pruning to Establish Correctly Polarized and Oriented Tip Links in Hair Bundles.Biophysical journal · 2017Article
- The Coupling between CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Efferent innervation of turtle semicircular canal cristae: comparisons with bird and mouse.The Journal of comparative neurology · 2015Article
- EF-hand protein Ca2+ buffers regulate Ca2+ influx and exocytosis in sensory hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Using the zebrafish lateral line to uncover novel mechanisms of action and prevention in drug-induced hair cell death.Frontiers in cellular neuroscience · 2015Review
- The physiology of mechanoelectrical transduction channels in hearing.Physiological reviews · 2014Review
- Disruption of intracellular calcium regulation is integral to aminoglycoside-induced hair cell death.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Electrical tuning and transduction in short hair cells of the chicken auditory papilla.Journal of neurophysiology · 2013Article
- Alterations of the CIB2 calcium- and integrin-binding protein cause Usher syndrome type 1J and nonsyndromic deafness DFNB48.Nature genetics · 2012Article
- Frequency-selective exocytosis by ribbon synapses of hair cells in the bullfrog's amphibian papilla.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells.The European journal of neuroscience · 2012Article
- New developments in understanding the mechanisms and function of spontaneous electrical activity in the developing mammalian auditory system.Journal of the Association for Research in Otolaryngology : JARO · 2012Review
- Calcium signaling in the cochlea - Molecular mechanisms and physiopathological implications.Cell communication and signaling : CCS · 2012Article
- Comparative examination of inner ear in wild type and pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice.Neurotoxicity research · 2012Article
- Distinct energy metabolism of auditory and vestibular sensory epithelia revealed by quantitative mass spectrometry using MS2 intensity.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Hair cells of the inner ear contain high concentrations of calcium-binding proteins that limit calcium signals and prevent cross talk between different signaling pathways during auditory transduction. Using light microscope immunofluorescence and post-embedding immunogold labeling in the electron microscope, we characterized the distribution of three calcium-buffering proteins in the turtle cochlea. Both calbindin-D28k and parvalbumin-beta were confined to hair cells in which they showed a similar distribution, whereas calretinin was present mainly in hair-cell nuclei but also occurred in supporting cells and nerve fibers. The hair-cell concentration of calbindin-D28k but not of parvalbumin-beta increased from the low- to high-frequency end of the cochlea. Calibration against standards containing known amounts of calcium-buffering protein processed in the same fluid drop as the cochlear sections gave cytoplasmic concentrations of calbindin-D28k as 0.13-0.63 mm and parvalbumin-beta as approximately 0.25 mm, but calretinin was an order of magnitude less. Total amount of Ca 2+-binding sites on the proteins is at least 1.0 mm in low-frequency hair cells and 3.0 mm in high-frequency cells. Reverse transcription-PCR showed that mRNA for all three proteins was expressed in turtle hair cells. We suggest that calbindin-D28k and parvalbumin-beta may serve as endogenous mobile calcium buffers, but the predominantly nuclear location of calretinin argues for another role in calcium signaling. The results support conclusions from electrophysiological measurements that millimolar concentrations of endogenous calcium buffers are present in turtle hair cells. Parvalbumin-beta was also found in both inner and outer hair cells of the guinea pig cochlea.
Indexed as
Identifiers
12805298PMC6740801W2125069729What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.