ArticleGenome research2003
Systematic cloning of Treponema pallidum open reading frames for protein expression and antigen discovery.
Article in Genome research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Profiling expression strategies for a type III polyketide synthase in a lysate-based, cell-free system.Scientific reports · 2024Article
- Genome analysis and hyphal movement characterization of the hitchhiker endohyphalApplied and environmental microbiology · 2024Article
- Characterizing the Syphilis-Causing Treponema pallidum ssp. pallidum Proteome Using Complementary Mass Spectrometry.PLoS neglected tropical diseases · 2016Article
- Contribution of a Comparative Western Blot Method to Early Postnatal Diagnosis of Congenital Syphilis.Clinical and vaccine immunology : CVI · 2016Article
- Review
- Article
- Genetic diversity in Treponema pallidum: implications for pathogenesis, evolution and molecular diagnostics of syphilis and yaws.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Whole genome sequences of three Treponema pallidum ssp. pertenue strains: yaws and syphilis treponemes differ in less than 0.2% of the genome sequence.PLoS neglected tropical diseases · 2012Article
- High throughput sequencing and proteomics to identify immunogenic proteins of a new pathogen: the dirty genome approach.PloS one · 2009Article
- Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins.Proteomics · 2009Article
- Generation and validation of a Shewanella oneidensis MR-1 clone set for protein expression and phage display.PloS one · 2008Article
- The ORFeome of Staphylococcus aureus v 1.1.BMC genomics · 2008Article
- Article
- A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin.Infection and immunity · 2008Article
- Identification of novel immunogens in Pasteurella multocida.Microbial cell factories · 2007Article
- The protein network of bacterial motility.Molecular systems biology · 2007Article
- Novel conserved assembly factor of the bacterial flagellum.Journal of bacteriology · 2006Article
- Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome.Journal of clinical microbiology · 2006Article
- Biological basis for syphilis.Clinical microbiology reviews · 2006Review
- Development of a functional genomics platform for Sinorhizobium meliloti: construction of an ORFeome.Applied and environmental microbiology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
A topoisomerase-based method was used to clone PCR products encoding 991 of the 1041 open reading frames identified in the genome sequence of the bacterium that causes syphilis, Treponema pallidum subsp. pallidum. Cloning the open reading frames into the univector plasmid system permitted the rapid conversion of the original clone set to other functional vectors containing a variety of promoters or tag sequences. A computational prediction of signal sequences identified 248 T. pallidum proteins that are potentially secreted from the cell. These clones were systematically converted into vectors designed to express the encoded proteins as glutathione-S-transferase fusion proteins. To test the potential of the clone set for novel antigen discovery, 85 of these fusion proteins were expressed from Escherichia coli, partially purified, and tested for antigenicity by using sera from rabbits infected with T. pallidum. Twelve of the 85 proteins bound significant levels of antibody. Of these 12 proteins, seven had previously been identified as T. pallidum antigens, and the remaining five represent novel antigens. These results demonstrate the potential of the T. pallidum clone set for antigen discovery and, more generally, for advancing the biology of this enigmatic spirochete.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.