ArticleThe EMBO journal2003
Truncated product of the bifunctional DLST gene involved in biogenesis of the respiratory chain.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Recurrent Germline DLST Mutations in Individuals with Multiple Pheochromocytomas and Paragangliomas.American journal of human genetics · 2019Article
- Association ofExperimental and therapeutic medicine · 2018Article
- The negative impact of α-ketoglutarate dehydrogenase complex deficiency on matrix substrate-level phosphorylation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Quantitative proteomic analysis in metastatic renal cell carcinoma reveals a unique set of proteins with potential prognostic significance.Molecular & cellular proteomics : MCP · 2013Article
- The application of ribozymes and DNAzymes in muscle and brain.Molecules (Basel, Switzerland) · 2010Review
- Cause and consequence: mitochondrial dysfunction initiates and propagates neuronal dysfunction, neuronal death and behavioral abnormalities in age-associated neurodegenerative diseases.Biochimica et biophysica acta · 2010Review
- Mutations in the mitochondrial genome confer resistance of cancer cells to anticancer drugs.Cancer science · 2009Article
- The alpha-ketoglutarate-dehydrogenase complex: a mediator between mitochondria and oxidative stress in neurodegeneration.Molecular neurobiology · 2005Review
- Catalytic nucleic acid enzymes for the study and development of therapies in the central nervous system: Review Article.Gene therapy & molecular biology · 2005Article
- Mitochondrial alpha-ketoglutarate dehydrogenase complex generates reactive oxygen species.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Mitochondrial dysfunction and oxidative damage in Alzheimer's and Parkinson's diseases and coenzyme Q10 as a potential treatment.Journal of bioenergetics and biomembranes · 2004Review
- Oxidative stress and transcriptional regulation in Alzheimer disease.Alzheimer disease and associated disordersReview
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dihydrolipoamide succinyltransferase (DLST) is a subunit enzyme of the alpha-ketoglutarate dehydrogenase complex of the Krebs cycle. While studying how the DLST genotype contributes to the pathogenesis of Alzheimer's disease (AD), we found a novel mRNA that is transcribed starting from intron 7 in the DLST gene. The novel mRNA level in the brain of AD patients was significantly lower than that of controls. The truncated gene product (designated MIRTD) localized to the intermembrane space of mitochondria. To investigate the function of MIRTD, we established human neuroblastoma SH-SY5Y cells expressing a maxizyme, a kind of ribozyme, that specifically digests the MIRTD mRNA. The expression of the maxizyme specifically eliminated the MIRTD protein and the resultant MIRTD-deficient cells exhibited a marked decrease in the amounts of subunits of complexes I and IV of the mitochondrial respiratory chain, resulting in a decline of activity. A pulse-label experiment revealed that the loss of the subunits is a post-translational event. Thus, the DLST gene is bifunctional and MIRTD transcribed from the gene contributes to the biogenesis of the mitochondrial respiratory complexes.
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