ArticleMolecular biology of the cell2003
Spindle formation in Aspergillus is coupled to tubulin movement into the nucleus.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Molecular insight into how γ-TuRC makes microtubules.Journal of cell science · 2021Review
- Anatomy of the fungal microtubule organizing center, the spindle pole body.Current opinion in structural biology · 2021Review
- Hydrodynamic Shape Changes Underpin Nuclear Rerouting in Branched Hyphae of an Oomycete Pathogen.mBio · 2019Article
- Tubulin is actively exported from the nucleus through the Exportin1/CRM1 pathway.Scientific reports · 2019Article
- Molecular basis of resistance to the microtubule-depolymerizing antitumor compound plocabulin.Scientific reports · 2018Article
- A nuclear contortionist: the mitotic migration ofMycology · 2018Review
- Mitotic nuclear pore complex segregation involves Nup2 inThe Journal of cell biology · 2017Article
- Location and functional analysis of the Aspergillus nidulans Aurora kinase confirm mitotic functions and suggest non-mitotic roles.Fungal genetics and biology : FG & B · 2017Article
- Mto2 multisite phosphorylation inactivates non-spindle microtubule nucleation complexes during mitosis.Nature communications · 2015Article
- Nup2 requires a highly divergent partner, NupA, to fulfill functions at nuclear pore complexes and the mitotic chromatin region.Molecular biology of the cell · 2015Article
- Restraint of the G2/M transition by the SR/RRM family mRNA shuttling binding protein SNXAHRB1 in Aspergillus nidulans.Genetics · 2014Article
- The Set1/COMPASS histone H3 methyltransferase helps regulate mitosis with the CDK1 and NIMA mitotic kinases in Aspergillus nidulans.Genetics · 2014Article
- Article
- The NIMA kinase is required to execute stage-specific mitotic functions after initiation of mitosis.Eukaryotic cell · 2014Article
- Article
- Localized accumulation of tubulin during semi-open mitosis in the Caenorhabditis elegans embryo.Molecular biology of the cell · 2012Article
- Nucleocytoplasmic shuttling of cytoskeletal proteins: molecular mechanism and biological significance.International journal of cell biology · 2012Article
- Nucleolar separation from chromosomes during Aspergillus nidulans mitosis can occur without spindle forces.Molecular biology of the cell · 2009Article
- Mlp1 acts as a mitotic scaffold to spatially regulate spindle assembly checkpoint proteins in Aspergillus nidulans.Molecular biology of the cell · 2009Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
In many important organisms, including many algae and most fungi, the nuclear envelope does not disassemble during mitosis. This fact raises the possibility that mitotic onset and/or exit might be regulated, in part, by movement of important mitotic proteins into and out of the nucleoplasm. We have used two methods to determine whether tubulin levels in the nucleoplasm are regulated in the fungus Aspergillus nidulans. First, we have used benomyl to disassemble microtubules and create a pool of free tubulin that can be readily observed by immunofluorescence. We find that tubulin is substantially excluded from interphase nuclei, but is present in mitotic nuclei. Second, we have observed a green fluorescent protein/alpha-tubulin fusion in living cells by time-lapse spinning-disk confocal microscopy. We find that tubulin is excluded from interphase nuclei, enters the nucleus seconds before the mitotic spindle begins to form, and is removed from the nucleoplasm during the M-to-G1 transition. Our data indicate that regulation of intranuclear tubulin levels plays an important, perhaps essential, role in the control of mitotic spindle formation in A. nidulans. They suggest that regulation of protein movement into the nucleoplasm may be important for regulating mitotic onset in organisms with intranuclear mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.