ArticleMolecular biology of the cell2003
MyoD distal regulatory region contains an SRF binding CArG element required for MyoD expression in skeletal myoblasts and during muscle regeneration.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 65 citations in OpenAlex.
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- Chromatin Landscape During Skeletal Muscle Differentiation.Frontiers in genetics · 2020Review
- Master control: transcriptional regulation of mammalian Myod.Journal of muscle research and cell motility · 2019Review
- MicroRNA-664-5p promotes myoblast proliferation and inhibits myoblast differentiation by targeting serum response factor andThe Journal of biological chemistry · 2018Article
- MUNC, an Enhancer RNA Upstream from theMolecular and cellular biology · 2018Article
- Srf controls satellite cell fusion through the maintenance of actin architecture.The Journal of cell biology · 2018Article
- Deltex2 represses MyoD expression and inhibits myogenic differentiation by acting as a negative regulator of Jmjd1c.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Myocardin-related transcription factors are required for skeletal muscle development.Development (Cambridge, England) · 2016Article
- Functions of the Tumor Suppressors p53 and Rb in Actin Cytoskeleton Remodeling.BioMed research international · 2016Review
- Association of lamin A/C with muscle gene-specific promoters in myoblasts.Biochemistry and biophysics reports · 2015Article
- Potential gene regulatory role for cyclin D3 in muscle cells.Journal of biosciences · 2015Article
- Expression of Non-acetylatable H2A.Z in Myoblast Cells Blocks Myoblast Differentiation through Disruption of MyoD Expression.The Journal of biological chemistry · 2015Article
- Age-related decreases of serum-response factor levels in human mesenchymal stem cells are involved in skeletal muscle differentiation and engraftment capacity.Stem cells and development · 2014Article
- G-protein coupled receptor 56 promotes myoblast fusion through serum response factor- and nuclear factor of activated T-cell-mediated signalling but is not essential for muscle development in vivo.The FEBS journal · 2013Article
- MicroRNA-199a is induced in dystrophic muscle and affects WNT signaling, cell proliferation, and myogenic differentiation.Cell death and differentiation · 2013Article
- miR-411 is up-regulated in FSHD myoblasts and suppresses myogenic factors.Orphanet journal of rare diseases · 2013Article
- Myod and H19-Igf2 locus interactions are required for diaphragm formation in the mouse.Development (Cambridge, England) · 2013Article
- Satellite cells and the muscle stem cell niche.Physiological reviews · 2013Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show here that the distal regulatory region (DRR) of the mouse and human MyoD gene contains a conserved SRF binding CArG-like element. In electrophoretic mobility shift assays with myoblast nuclear extracts, this CArG sequence, although slightly divergent, bound two complexes containing, respectively, the transcription factor YY1 and SRF associated with the acetyltransferase CBP and members of C/EBP family. A single nucleotide mutation in the MyoD-CArG element suppressed binding of both SRF and YY1 complexes and abolished DRR enhancer activity in stably transfected myoblasts. This MyoD-CArG sequence is active in modulating endogeneous MyoD gene expression because microinjection of oligonucleotides corresponding to the MyoD-CArG sequence specifically and rapidly suppressed MyoD expression in myoblasts. In vivo, the expression of a transgenic construct comprising a minimal MyoD promoter fused to the DRR and beta-galactosidase was induced with the same kinetics as MyoD during mouse muscle regeneration. In contrast induction of this reporter was no longer seen in regenerating muscle from transgenic mice carrying a mutated DRR-CArG. These results show that an SRF binding CArG element present in MyoD gene DRR is involved in the control of MyoD gene expression in skeletal myoblasts and in mature muscle satellite cell activation during muscle regeneration.
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