ArticleMolecular biology of the cell2003
A novel dynein light intermediate chain colocalizes with the retrograde motor for intraflagellar transport at sites of axoneme assembly in chlamydomonas and Mammalian cells.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 132 citations in OpenAlex.
- Changes in the Adenylate Kinase Activity are Proportional to the ADP/ATP Ratio Upon Resorption and Regeneration of Chlamydomonas reinhardtii Flagella.Cell biochemistry and biophysics · 2025Article
- Roles for CEP170 in cilia function and dynein-2 assembly.Journal of cell science · 2024Article
- The Logistical Backbone of Photoreceptor Cell Function: Complementary Mechanisms of Dietary Vitamin A Receptors and Rhodopsin Transporters.International journal of molecular sciences · 2024Review
- Appearing and disappearing acts of cilia.Journal of biosciences · 2023Review
- DYNC1LI2 regulates localization of the chaperone-mediated autophagy receptor LAMP2A and improves cellular homeostasis in cystinosis.Autophagy · 2022Article
- Polycystin-2 (TRPP2) regulates primary cilium length in LLC-PK1 renal epithelial cells.Frontiers in physiology · 2022Article
- In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip.Journal of cell science · 2021Article
- Ciliary Dyneins and Dynein Related Ciliopathies.Cells · 2021Review
- The pseudoGTPase group of pseudoenzymes.The FEBS journal · 2020Review
- Lis1 activates dynein motility by modulating its pairing with dynactin.Nature cell biology · 2020Article
- Non-ciliary Roles of IFT Proteins in Cell Division and Polycystic Kidney Diseases.Frontiers in cell and developmental biology · 2020Review
- FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia.Molecular biology of the cell · 2019Article
- The role of retrograde intraflagellar transport genes in aminoglycoside-induced hair cell death.Biology open · 2019Article
- Auto-fatty acylation of transcription factor RFX3 regulates ciliogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Emerging mechanisms of dynein transport in the cytoplasm versus the cilium.Biochemical Society transactions · 2018Review
- Article
- Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery.The FEBS journal · 2017Review
- Intraflagellar Transport and Ciliary Dynamics.Cold Spring Harbor perspectives in biology · 2017Review
- Ensemble and single-molecule dynamics of IFT dynein in Caenorhabditis elegans cilia.Nature communications · 2017Article
- The Intraflagellar Transport Machinery.Cold Spring Harbor perspectives in biology · 2016Review
6 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The assembly of cilia and flagella depends on bidirectional intraflagellar transport (IFT). Anterograde IFT is driven by kinesin II, whereas retrograde IFT requires cytoplasmic dynein 1b (cDHC1b). Little is known about how cDHC1b interacts with its cargoes or how it is regulated. Recent work identified a novel dynein light intermediate chain (D2LIC) that colocalized with the mammalian cDHC1b homolog DHC2 in the centrosomal region of cultured cells. To see whether the LIC might play a role in IFT, we characterized the gene encoding the Chlamydomonas homolog of D2LIC and found its expression is up-regulated in response to deflagellation. We show that the LIC subunit copurifies with cDHC1b during flagellar isolation, dynein extraction, sucrose density centrifugation, and immunoprecipitation. Immunocytochemistry reveals that the LIC colocalizes with cDHC1b in the basal body region and along the length of flagella in wild-type cells. Localization of the complex is altered in a collection of retrograde IFT and length control mutants, which suggests that the affected gene products directly or indirectly regulate cDHC1b activity. The mammalian DHC2 and D2LIC also colocalize in the apical cytoplasm and axonemes of ciliated epithelia in the lung, brain, and efferent duct. These studies, together with the identification of an LIC mutation, xbx-1(ok279), which disrupts retrograde IFT in Caenorhabditis elegans, indicate that the novel LIC is a component of the cDHC1b/DHC2 retrograde IFT motor in a variety of organisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.