ArticleMolecular biology of the cell2003
Centrosomes split in the presence of impaired DNA integrity during mitosis.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 130 citations in OpenAlex.
- CenSegNet: a generalist high-throughput deep learning framework for centrosome phenotyping at spatial and single-cell resolution in heterogeneous tissues.Nature communications · 2026Article
- Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.Cell death and differentiation · 2023Article
- Intrinsic neural stem cell properties define brain hypersensitivity to genotoxic stress.Stem cell reports · 2022Article
- Centrosome amplification: a quantifiable cancer cell trait with prognostic value in solid malignancies.Cancer metastasis reviews · 2021Review
- Article
- Targeting the Id1-Kif11 Axis in Triple-Negative Breast Cancer Using Combination Therapy.Biomolecules · 2020Article
- Review
- A guiding torch at the poles: the multiple roles of spindle microtubule-organizing centers during cell division.Cell cycle (Georgetown, Tex.) · 2020Review
- CSAG1 maintains the integrity of the mitotic centrosome in cells with defective p53.Journal of cell science · 2020Article
- Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites.Molecular and cellular biology · 2018Article
- The Replication Protein Cdc6 Suppresses Centrosome Over-Duplication in a Manner Independent of Its ATPase Activity.Molecules and cells · 2017Article
- Article
- 14-3-3γ Prevents Centrosome Amplification and Neoplastic Progression.Scientific reports · 2016Article
- Centrosomes are multifunctional regulators of genome stability.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Review
- A novel role for the deubiquitinase USP1 in the control of centrosome duplication.Cell cycle (Georgetown, Tex.) · 2016Article
- Centrosomes in the DNA damage response--the hub outside the centre.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Review
- Dynein light intermediate chains maintain spindle bipolarity by functioning in centriole cohesion.The Journal of cell biology · 2014Article
- Centrosomes as signalling centres.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Mitotic spindle multipolarity without centrosome amplification.Nature cell biology · 2014Review
- Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A well-established function of centrosomes is their role in accomplishing a successful mitosis that gives rise to a pair of identical daughter cells. We recently showed that DNA replication defects and DNA damage in Drosophila embryos trigger centrosomal changes, but it remained unclear whether comparable centrosomal responses can be provoked in somatic mammalian cells. To investigate the centrosomal organization in the presence of impaired DNA integrity, live and ultrastructural analysis was performed on gamma-tubulin-GFP and EGFP-alpha-tubulin-expressing Chinese hamster ovary cells. We have shown that during mitosis in the presence of incompletely replicated or damaged DNA, centrosomes split into fractions containing only one centriole. This results in the formation of multipolar spindles with extra centrosome-like structures. Despite the extra centrosomes and the multipolarity of the spindles, cells do exit from mitosis, resulting in severe division errors. Our data provide evidence of a novel mechanism showing how numerous centrosomes and spindle defects can arise and how this can lead to the formation of aneuploid cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.