ArticleMolecular biology of the cell2003
Actin dynamics is controlled by a casein kinase II and phosphatase 2C interplay on Toxoplasma gondii Toxofilin.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- Deciphering the Role of Protein Phosphatases in Apicomplexa: The Future of Innovative Therapeutics?Microorganisms · 2022Review
- Parasite protein phosphatases: biological function, virulence, and host immune evasion.Parasitology research · 2021Review
- Protein kinase CK2: a potential therapeutic target for diverse human diseases.Signal transduction and targeted therapy · 2021Review
- The catalytic subunit of Plasmodium falciparum casein kinase 2 is essential for gametocytogenesis.Communications biology · 2021Article
- iTRAQ-based comparative proteomic analysis in different developmental stages of Echinococcus granulosus.Parasite (Paris, France) · 2021Article
- TheFrontiers in cellular and infection microbiology · 2021Article
- iTRAQ-Based Global Phosphoproteomics Reveals Novel Molecular Differences BetweenFrontiers in cellular and infection microbiology · 2019Article
- The serine/threonine phosphatases of apicomplexan parasites.Molecular microbiology · 2017Review
- Genetic impairment of parasite myosin motors uncovers the contribution of host cell membrane dynamics to Toxoplasma invasion forces.BMC biology · 2016Article
- An RNA-binding protein, RNP-1, protects microtubules from nocodazole and localizes to the leading edge during cytokinesis and cell migration in Dictyostelium cells.Acta pharmacologica Sinica · 2016Article
- Host cell invasion by apicomplexan parasites: the junction conundrum.PLoS pathogens · 2014Review
- Specific phosphorylation of the PfRh2b invasion ligand of Plasmodium falciparum.The Biochemical journal · 2013Article
- Toxofilin upregulates the host cortical actin cytoskeleton dynamics, facilitating Toxoplasma invasion.Journal of cell science · 2012Article
- Quantitative in vivo analyses reveal calcium-dependent phosphorylation sites and identifies a novel component of the Toxoplasma invasion motor complex.PLoS pathogens · 2011Article
- A highly sensitive FRET-based approach reveals secretion of the actin-binding protein toxofilin during Toxoplasma gondii infection.Cellular microbiology · 2010Article
- Effects of calcium signaling on Plasmodium falciparum erythrocyte invasion and post-translational modification of gliding-associated protein 45 (PfGAP45).Molecular and biochemical parasitology · 2009Article
- piggyBac is an effective tool for functional analysis of the Plasmodium falciparum genome.BMC microbiology · 2009Article
- GAP45 phosphorylation controls assembly of the Toxoplasma myosin XIV complex.Eukaryotic cell · 2009Article
- Toxofilin from Toxoplasma gondii forms a ternary complex with an antiparallel actin dimer.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A Toxoplasma gondii leucine-rich repeat protein binds phosphatase type 1 protein and negatively regulates its activity.Eukaryotic cell · 2007Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin polymerization in Apicomplexa protozoa is central to parasite motility and host cell invasion. Toxofilin has been characterized as a protein that sequesters actin monomers and caps actin filaments in Toxoplasma gondii. Herein, we show that Toxofilin properties in vivo as in vitro depend on its phosphorylation. We identify a novel parasitic type 2C phosphatase that binds the Toxofilin/G-actin complex and a casein kinase II-like activity in the cytosol, both of which modulate the phosphorylation status of Toxofilin serine53. The interplay of these two molecules controls Toxofilin binding of G-actin as well as actin dynamics in vivo. Such functional interactions should play a major role in actin sequestration, a central feature of actin dynamics in Apicomplexa that underlies the spectacular speed and nature of parasite gliding motility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.