ArticleMolecular biology of the cell2003
The AP-1 clathrin-adaptor is required for lysosomal enzymes sorting and biogenesis of the contractile vacuole complex in Dictyostelium cells.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- The AP-1 adaptor complex is required for cell surface modifications and the survival ofInfection and immunity · 2026Article
- GRASP55 maintains lysosome function by controlling sorting of lysosomal enzymes at the Golgi.EMBO reports · 2026Article
- The Polarized Redistribution of the Contractile Vacuole to the Rear of the Cell is Critical for Streaming and is Regulated by PI(4,5)P2-Mediated Exocytosis.Frontiers in cell and developmental biology · 2021Review
- Membrane Compartmentalization and Scaffold Proteins in Leukocyte Migration.Frontiers in cell and developmental biology · 2020Review
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- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Dynamics of clathrin-mediated endocytosis and its requirement for organelle biogenesis in Dictyostelium.Journal of cell science · 2012Article
- Expression and localization of silkworm adaptor protein complex-1 subunits, which were down-regulated post baculovirus infection.Molecular biology reports · 2012Article
- CP55, a novel key component of centrosomal organization in Dictyostelium.Cellular and molecular life sciences : CMLS · 2012Article
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- A single β adaptin contributes to AP1 and AP2 complexes and clathrin function in Dictyostelium.Traffic (Copenhagen, Denmark) · 2012Article
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- Roles of an unconventional protein kinase and myosin II in amoeba osmotic shock responses.Traffic (Copenhagen, Denmark) · 2009Article
- AP180-mediated trafficking of Vamp7B limits homotypic fusion of Dictyostelium contractile vacuoles.Molecular biology of the cell · 2009Article
- Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development.Molecular biology of the cell · 2008Article
- Leishmania adaptor protein-1 subunits are required for normal lysosome traffic, flagellum biogenesis, lipid homeostasis, and adaptation to temperatures encountered in the mammalian host.Eukaryotic cell · 2008Article
- Identification and characterization of a novel alpha-kinase with a von Willebrand factor A-like motif localized to the contractile vacuole and Golgi complex in Dictyostelium discoideum.Molecular biology of the cell · 2005Article
- Involvement of the AP-1 adaptor complex in early steps of phagocytosis and macropinocytosis.Molecular biology of the cell · 2004Article
- Resolving the homology-function relationship through comparative genomics of membrane-trafficking machinery and parasite cell biology.Molecular and biochemical parasitologyReview
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Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adaptor protein complexes (AP) are major components of the cytoplasmic coat found on clathrin-coated vesicles. Here, we report the molecular and functional characterization of Dictyostelium clathrin-associated AP-1 complex, which in mammalian cells, participates mainly in budding of clathrin-coated vesicles from the trans-Golgi network (TGN). The gamma-adaptin AP-1 subunit was cloned and shown to belong to a Golgi-localized 300-kDa protein complex. Time-lapse analysis of cells expressing gamma-adaptin tagged with the green-fluorescent protein demonstrates the dynamics of AP-1-coated structures leaving the Golgi apparatus and rarely moving toward the TGN. Targeted disruption of the AP-1 medium chain results in viable cells displaying a severe growth defect and a delayed developmental cycle compared with parental cells. Lysosomal enzymes are constitutively secreted as precursors, suggesting that protein transport between the TGN and lysosomes is defective. Although endocytic protein markers are correctly localized to endosomal compartments, morphological and ultrastructural studies reveal the absence of large endosomal vacuoles and an increased number of small vacuoles. In addition, the function of the contractile vacuole complex (CV), an osmoregulatory organelle is impaired and some CV components are not correctly targeted.
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