ArticleMolecular biology of the cell2003
NZO-3 expression causes global changes to actin cytoskeleton in Madin-Darby canine kidney cells: linking a tight junction protein to Rho GTPases.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- The multifarious regulation of the apical junctional complex.Open biology · 2020Review
- Zonula occludens-1 and -2 regulate apical cell structure and the zonula adherens cytoskeleton in polarized epithelia.Molecular biology of the cell · 2012Article
- Regulation of epithelial permeability by the actin cytoskeleton.Cytoskeleton (Hoboken, N.J.) · 2011Review
- Tight function zonula occludens-3 regulates cyclin D1-dependent cell proliferation.Molecular biology of the cell · 2011Article
- A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and adherens junctions of epithelial cells.The Journal of biological chemistry · 2011Article
- New aspects of the molecular constituents of tissue barriers.Journal of neural transmission (Vienna, Austria : 1996) · 2011Review
- Evolution of the MAGUK protein gene family in premetazoan lineages.BMC evolutionary biology · 2010Article
- The dual role of zonula occludens (ZO) proteins.Journal of biomedicine & biotechnology · 2010Review
- Zonula occludens-1 and -2 are cytosolic scaffolds that regulate the assembly of cellular junctions.Annals of the New York Academy of Sciences · 2009Review
- Adherens and tight junctions: structure, function and connections to the actin cytoskeleton.Biochimica et biophysica acta · 2008Review
- The NHERF1 PDZ2 domain regulates PKA-RhoA-p38-mediated NHE1 activation and invasion in breast tumor cells.Molecular biology of the cell · 2007Article
- Normal establishment of epithelial tight junctions in mice and cultured cells lacking expression of ZO-3, a tight-junction MAGUK protein.Molecular and cellular biology · 2006Article
- Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.Molecular biology of the cell · 2006Article
- Association with ZO-1 correlates with plasma membrane partitioning in truncated connexin45 mutants.The Journal of membrane biology · 2005Article
- Protein kinase A gating of a pseudopodial-located RhoA/ROCK/p38/NHE1 signal module regulates invasion in breast cancer cell lines.Molecular biology of the cell · 2005Article
- The Na+-H+ exchanger-1 induces cytoskeletal changes involving reciprocal RhoA and Rac1 signaling, resulting in motility and invasion in MDA-MB-435 cells.Breast cancer research : BCR · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously demonstrated that exogenous expression of a truncated form of the tight junction protein ZO-3 affected junctional complex assembly and function. Current results indicate that this ZO-3 construct influences actin cytoskeleton dynamics more globally. We show that expression of the amino-terminal half of ZO-3 (NZO-3) in Madin-Darby canine kidney cells results in a decreased number of stress fibers and focal adhesions and causes an increased rate of cell migration in a wound healing assay. We also demonstrate that RhoA activity is reduced in NZO-3-expressing cells. We determined that ZO-3 interacts with p120 catenin and AF-6, proteins localized to the junctional complex and implicated in signaling pathways important for cytoskeleton regulation and cell motility. We also provide evidence that NZO-3 interacts directly with the C terminus of ZO-3, and we propose a model where altered interactions between ZO-3 and p120 catenin in NZO-3-expressing cells affect RhoA GTPase activity. This study reveals a potential link between ZO-3 and RhoA-related signaling events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.