ArticleProceedings of the National Academy of Sciences of the United States of America2003
Carbohydrate-dependent signaling from the phosphatidylglucoside-based microdomain induces granulocytic differentiation of HL60 cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 64 citations in OpenAlex.
- Phosphatidylglucoside regulates apoptosis of human neutrophilic lineage cells.Frontiers in immunology · 2025Article
- Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages.Scientific reports · 2023Article
- Multiplicity of Glycosphingolipid-Enriched Microdomain-Driven Immune Signaling.International journal of molecular sciences · 2021Review
- Exogenous phosphatidylglucoside alleviates cognitive impairment by improvement of neuroinflammation, and neurotrophin signaling.Clinical and translational medicine · 2021Article
- Glycolipids: Linchpins in the Organization and Function of Membrane Microdomains.Frontiers in cell and developmental biology · 2020Review
- Sphingolipids in cardiovascular and cerebrovascular systems: Pathological implications and potential therapeutic targets.World journal of cardiology · 2013Article
- A world of sphingolipids and glycolipids in the brain--novel functions of simple lipids modified with glucose.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2012Review
- Stem cell glycolipids.Neurochemical research · 2011Review
- Secretagogin is a Ca2+-binding protein identifying prospective extended amygdala neurons in the developing mammalian telencephalon.The European journal of neuroscience · 2010Article
- Fucoganglioside alpha-fucosyl(alpha-galactosyl)-GM1: a novel member of lipid membrane microdomain components involved in PC12 cell neuritogenesis.The Biochemical journal · 2007Article
- Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling.Molecular biology of the cell · 2007Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosphingolipids form glycosphingolipid signaling microdomains. Here, we report an unrecognized type of phosphatidylglucoside (PhGlc)-based lipid microdomain in HL60 cells. Treatment of cells with rGL-7, which preferentially reacts with PhGlc, induced differentiation of HL60 cells. This was manifested by the appearance of nitroblue tetrazolium-positive cells together with CD38 expression and c-Myc down-regulation. We determined the molecular mechanisms underlying early stages of signal transduction. rGL-7 treatment induced rapid tyrosine phosphorylation of Src family protein kinases Lyn and Hck. Reduction of endogenous cholesterol after application of methyl-beta-cyclodextrin suppressed rGL-7-stimulated tyrosine phosphorylation. Phosphorylated proteins and PhGlc colocalized in the Triton X-100 insoluble, light buoyant density fraction after sucrose gradient ultracentrifugation of HL60 cell lysates. This suggests PhGlc-based microdomain is involved in GL-7 signaling. Ligation of known components of microdomains, such as sphingomyelin and ganglioside GM1, with corresponding antibodies failed to induce differentiation and tyrosine phosphorylation. These results show that PhGlc constitutes a previously undescribed lipid signaling domain, and the glucose residue of PhGlc is critical for organization of the carbohydrate-dependent signaling domain involved in cellular differentiation of HL60 cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.