ArticleProceedings of the National Academy of Sciences of the United States of America2003
Stepwise cytoskeletal polarization as a series of checkpoints in innate but not adaptive cytolytic killing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Neutral sphingomyelinases restrict natural killer cells activity against lung cancer.Cancer immunology, immunotherapy : CII · 2026Article
- Spatial localization of CD16a at the human NK cell ADCC lytic synapse.bioRxiv : the preprint server for biology · 2024Article
- Human natural killer cells: Form, function, and development.The Journal of allergy and clinical immunology · 2023Review
- Horses for Courses in the Era of CARs: Advancing CAR T and CAR NK Cell Therapies.Journal of personalized medicine · 2021Review
- NK Cells in aFrontiers in immunology · 2021Review
- The Role of the Cytoskeleton in Regulating the Natural Killer Cell Immune Response in Health and Disease: From Signaling Dynamics to Function.Frontiers in cell and developmental biology · 2021Review
- Filamin A Is Required for NK Cell Cytotoxicity at the Expense of Cytokine ProductionFrontiers in immunology · 2021Article
- Treating primary immunodeficiencies with defects in NK cells: from stem cell therapy to gene editing.Stem cell research & therapy · 2020Review
- PD-1 suppresses the maintenance of cell couples between cytotoxic T cells and target tumor cells within the tumor.Science signaling · 2020Article
- The DISC1-Girdin complex - a missing link in signaling to the T cell cytoskeleton.Journal of cell science · 2020Article
- Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells.Current biology : CB · 2018Article
- Phosphoinositide-3-Kinase Signaling in Human Natural Killer Cells: New Insights from Primary Immunodeficiency.Frontiers in immunology · 2018Review
- Quantitative Imaging Approaches to Study the CAR Immunological Synapse.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Review
- Fas ligand and lytic granule differentially control cytotoxic dynamics of natural killer cell against cancer target.Oncotarget · 2016Article
- UNC-45A Is a Nonmuscle Myosin IIA Chaperone Required for NK Cell Cytotoxicity via Control of Lytic Granule Secretion.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- NKp46 Clusters at the Immune Synapse and Regulates NK Cell Polarization.Frontiers in immunology · 2015Article
- Lytic immune synapse function requires filamentous actin deconstruction by Coronin 1A.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Cell biological steps and checkpoints in accessing NK cell cytotoxicity.Immunology and cell biology · 2014Review
- The kinematics of cytotoxic lymphocytes influence their ability to kill target cells.PloS one · 2014Article
- Secretagogue stimulation of neurosecretory cells elicits filopodial extensions uncovering new functional release sites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytolytic killing is a major effector mechanism in the elimination of virally infected and tumor cells. The innate cytolytic effectors, natural killer (NK) cells, and the adaptive effectors, cytotoxic T cells (CTL), despite differential immune recognition, both use the same lytic mechanism, cytolytic granule release. Using live cell video fluorescence microscopy in various primary cell models of NK cell and CTL killing, we show here that on tight target cell contact, a majority of the NK cells established cytoskeletal polarity required for effective lytic function slowly or incompletely. In contrast, CTLs established cytoskeletal polarity rapidly. In addition, NK cell killing was uniquely sensitive to minor interference with cytoskeletal dynamics. We propose that the stepwise NK cell cytoskeletal polarization constitutes a series of checkpoints in NK cell killing. In addition, the use of more deliberate progression to effector function to compensate for inferior immune recognition specificity provides a mechanistic explanation for how the same effector function can be used in the different functional contexts of the innate and adaptive immune response.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.