ArticleGenome biology2003
A phylogenetic study of cytochrome b561 proteins.
Article in Genome biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
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- Iron Homeostasis in Insects.Annual review of entomology · 2023Review
- Comparison of insect and human cytochrome b561 proteins: Insights into candidate ferric reductases in insects.PloS one · 2023Article
- Lysosomal iron recycling in mouse macrophages is dependent upon both LcytB and Steap3 reductases.Blood advances · 2022Article
- Comparative Transcriptomics and Co-Expression Networks Reveal Tissue- and Genotype-Specific Responses ofGenes · 2020Article
- Drosophila melanogaster Models of Metal-Related Human Diseases and Metal Toxicity.International journal of molecular sciences · 2017Review
- ZIP13: A Study ofFrontiers in genetics · 2017Review
- Expression profile of Caenorhabditis elegans mutant for the Werner syndrome gene ortholog reveals the impact of vitamin C on development to increase life span.BMC genomics · 2014Article
- Structure and mechanism of a eukaryotic transmembrane ascorbate-dependent oxidoreductase.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Characterization of the Pinus massoniana transcriptional response to Bursaphelenchus xylophilus infection using suppression subtractive hybridization.International journal of molecular sciences · 2013Article
- Dihydrolipoic acid reduces cytochrome b561 proteins.European biophysics journal : EBJ · 2013Article
- Molecular organization of Drosophila neuroendocrine cells by Dimmed.Current biology : CB · 2011Article
- A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum.PLoS neglected tropical diseases · 2010Article
- More than 1,001 problems with protein domain databases: transmembrane regions, signal peptides and the issue of sequence homology.PLoS computational biology · 2010Article
- Spectral characterization of the recombinant mouse tumor suppressor 101F6 protein.European biophysics journal : EBJ · 2010Article
- Tuning of the thermochemical and kinetic properties of ascorbate by its local environment: solution chemistry and biochemical implications.Journal of the American Chemical Society · 2010Article
- Mining Cytochrome b561 proteins from plant genomes.International journal of bioinformatics research and applications · 2010Article
- Auxin-responsive genes AIR12 code for a new family of plasma membrane b-type cytochromes specific to flowering plants.Plant physiology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAs an antioxidant and cofactor to numerous metabolic enzymes, ascorbate has an essential role in plants and animals. Cytochromes b561 constitute a class of intrinsic membrane proteins involved in ascorbate regeneration. Despite their importance in ascorbate metabolism, no evolutionary analysis has been presented so far on this newly described protein family.
resultsCytochromes b561 have been identified in a large number of phylogenetically distant species, but are absent in fungi and prokaryotes. Most species contain three or four cytochrome b561 paralogous proteins, and the encoding genes usually have four or five exons. At the protein level, sequence similarities are rather low between cytochromes b561 within a single species (34-45% identity), and among phylogenetically distant species (around 30% identity). However, particular structural features characterizing this protein family are well conserved in members from all species investigated. These features comprise six transmembrane helices, four strictly conserved histidine residues, probably coordinating the two heme molecules, and putative ascorbate and monodehydro-ascorbate (MDHA) substrate-binding sites. Analysis of plant cytochromes b561 shows a separation between those from monocotyledonous and dicotyledonous species in a phylogenetic tree.
conclusionsAll cytochromes b561 have probably evolved from a common ancestral protein before the separation of plants and animals. Their phyletic distribution mirrors the use of ascorbate as primary antioxidant, indicating their role in ascorbate homeostasis and antioxidative defense. In plants, the differentiation into four cytochrome b561 isoforms probably occurred before the separation between monocots and dicots.
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