ArticleThe Biochemical journal1992
Studies on epitopes on low-density lipoprotein modified by 4-hydroxynonenal. Biochemical characterization and determination.
Article in The Biochemical journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 62 citations in OpenAlex.
- OxLDL-induced ferroptosis and pyroptosis in atherosclerosis: a mini review.Frontiers in immunology · 2026Review
- The 4-Hydroxynonenal-Protein Adducts and Their Biological Relevance: Are Some Proteins Preferred Targets?Antioxidants (Basel, Switzerland) · 2023Review
- Oxidative Stress in Autism Spectrum Disorder-Current Progress of Mechanisms and Biomarkers.Frontiers in psychiatry · 2022Review
- Lipoproteins as targets and markers of lipoxidation.Redox biology · 2019Review
- Lipoxidation in cardiovascular diseases.Redox biology · 2019Review
- DNA damage by lipid peroxidation products: implications in cancer, inflammation and autoimmunity.AIMS genetics · 2017Review
- The lipid peroxidation product 4-hydroxy-2-nonenal: Advances in chemistry and analysis.Redox biology · 2013Review
- Urinary biomarkers of oxidative status.Clinica chimica acta; international journal of clinical chemistry · 2012Review
- Application of medical and analytical methods in Lyme borreliosis monitoring.Analytical and bioanalytical chemistry · 2012Review
- trans,trans-2,4-decadienal induces mitochondrial dysfunction and oxidative stress.Journal of bioenergetics and biomembranes · 2008Article
- Anti-oxidized low-density lipoprotein antibodies in myeloperoxidase-positive vasculitis patients preferentially recognize hypochlorite-modified low density lipoproteins.Clinical and experimental immunology · 2007Article
- Did the antioxidant trials fail to validate the oxidation hypothesis?Current atherosclerosis reports · 2001Review
- Endogenously produced lipoprotein lipase enhances the binding and cell association of native, mildly oxidized and moderately oxidized low-density lipoprotein in mouse peritoneal macrophages.The Biochemical journal · 1999Article
- Oxidation of high-density lipoprotein HDL3 leads to exposure of apo-AI and apo-AII epitopes and to formation of aldehyde protein adducts, and influences binding of oxidized low-density lipoprotein to type I and type III collagen in vitro1.The Biochemical journal · 1998Article
- Formation of 4-hydroxy-2-nonenal-modified proteins in the renal proximal tubules of rats treated with a renal carcinogen, ferric nitrilotriacetate.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Immunochemical detection of 4-hydroxynonenal protein adducts in oxidized hepatocytes.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxidation of human low-density lipoprotein (LDL) was found to be accompanied by the generation of various reactive aldehydes. One of them, 4-hydroxynonenal (HNE), was shown to modify LDL to a form which represents a good model of oxidized LDL (ox-LDL). In order to investigate the epitopes newly formed on HNE-modified LDL, a polyvalent antiserum to HNE-LDL [anti-(HNE-LDL)] was raised in rabbits and the non-specific components were removed with native LDL coupled to CNBr-Sepharose 4B. Competitive fluorescence immunoassay analysis showed that anti-(HNE-LDL) recognized HNE-LDL, copper-oxidized LDL, HNE-albumin and to a lower extent HNE-modified high-density lipoprotein 3 (HNE-HDL3) and ox-HDL3 but not native LDL. A certain degree of cross-reactivity of the antibody with LDLs modified by either hexanal or 2,4-heptadienal was found. No reaction was obtained with LDL labelled with malondialdehyde. From the abilities of HNE-modified poly(L-amino acids) to compete with HNE-LDL for binding to anti-(HNE-LDL), it is postulated that lysine, tyrosine, arginine and histidine are involved in the formation of HNE-derived epitopes on apolipoprotein B (apo B). Using a double-sandwich fluorescence immunoassay [capture antibody: anti-(apo B); detection antibody: anti-(HNE-LDL)] we found that the HNE-derived epitopes were expressed at a far higher degree in ox-LDL and HNE-LDL than in native LDL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.