ArticleFolia microbiologica2003
Primers ITS1, ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi.
Article in Folia microbiologica, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
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- Biotechnological Potential of a Novel Strain of Fusarium proliferatum, a Terrestrial Fungus Adapted to Marine Environment.Environmental microbiology reports · 2025Article
- The Biotherapeutic Potential of a Novel ProbioticJournal of fungi (Basel, Switzerland) · 2025Article
- On-orbit microbial succession patterns of the China Space Station during the construction period.Microbiome · 2025Article
- Ventilator-associated pneumonia caused by a new-found opportunistic fungal pathogen-Myceliophthora heterothallica: a case report.BMC geriatrics · 2024Article
- Screening and Genomic Analysis of Alkaloid-Producing Endophytic FungusMicroorganisms · 2024Article
- Evaluation of Potential Probiotic Properties and In Vivo Safety of Lactic Acid Bacteria and Yeast Strains Isolated from Traditional Home-Made Kefir.Foods (Basel, Switzerland) · 2024Article
- Oxygen-dependent biofilm dynamics in leaf decay: an in vitro analysis.Scientific reports · 2024Article
- Two Cases of Superficial Fungal Infection Caused by Non-AlbicansInfection and drug resistance · 2024Article
- Microbiome in a ground-based analog cabin of China Space Station during a 50-day human occupation.ISME communications · 2024Article
- Decay experiments and microbial community analysis of water lily leaf biofilms: Sediment effects on leaf preservation potential.PloS one · 2024Article
- Evaluation of biocontrol efficacy of rhizosphere Pseudomonas aeruginosa for management of Phytophthora capsici of pepper.PloS one · 2024Article
- From Child to Old Man: A Slowly Evolving Case of Chromoblastomycosis Caused byAntibiotics (Basel, Switzerland) · 2023Article
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- Characterization and transcriptomic analysis of a native fungal pathogen against the rice pestFrontiers in microbiology · 2023Article
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- Fungal Invasive Co-Infection Due toJournal of fungi (Basel, Switzerland) · 2021Article
- Application of laser capture microdissection and PCR sequencing in the diagnosis ofEmerging microbes & infections · 2021Review
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Restriction fragment length polymorphism analysis of the 5.8S rRNA gene and the internal transcribed spacers (ITS1 and ITS2) was used for examination of 66 isolates belonging to 19 species. Intraspecies variability was found in the examined region of 11 species (Candida albicans, C. catenulata, C. colliculosa, C. glabrata, C. kefyr, C. melinii, C. parapsilosis, C. guillermondii, C. solanii, C. tropicalis, Saccharomyces cerevisiae). Region of ITS-5.8S rDNA was amplified using the primers ITS1 and ITS4. The amplicons were digested by HaeIII, HinfI and CfoI. The recognized intraspecies variability was confirmed in the second step, in which the shorter fragments of this region were amplified using primers ITS1 and ITS2 and analyzed by capillary electrophoresis. Considerable intraspecific variability renders this method unsuitable for species identification, whereas it can be useful for epidemiological tracing of isolates.
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