ArticleNucleic acids research2003
Conditional human VEGF-mediated vascularization in chicken embryos using a novel temperature-inducible gene regulation (TIGR) system.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- Synthetic biology approaches to generate temperature-sensitive alleles for the Sterile Insect Technique.Insect science · 2026Review
- Mapping temperature-sensitive mutations at a genome scale to engineer growth switches in Escherichia coli.Molecular systems biology · 2023Article
- Genetically Encoded Protein Thermometer Enables Precise Electrothermal Control of Transgene Expression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- High-throughput enrichment of temperature-sensitive argininosuccinate synthetase for two-stage citrulline production in E. coli.Metabolic engineering · 2020Article
- Review
- Light-Controlled Mammalian Cells and Their Therapeutic Applications in Synthetic Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Review
- The Promoter Toolbox for Recombinant Gene Expression inFrontiers in bioengineering and biotechnology · 2018Review
- Designed cell consortia as fragrance-programmable analog-to-digital converters.Nature chemical biology · 2017Article
- Cosmetics-triggered percutaneous remote control of transgene expression in mice.Nucleic acids research · 2015Article
- Mammalian synthetic biology: emerging medical applications.Journal of the Royal Society, Interface · 2015Review
- Synthetic biology: applications come of age.Nature reviews. Genetics · 2010Review
- A synthetic mammalian electro-genetic transcription circuit.Nucleic acids research · 2009Article
- Intronically encoded siRNAs improve dynamic range of mammalian gene regulation systems and toggle switch.Nucleic acids research · 2008Article
- Adeno-associated viral vectors engineered for macrolide-adjustable transgene expression in mammalian cells and mice.BMC biotechnology · 2007Article
- An engineered L-arginine sensor of Chlamydia pneumoniae enables arginine-adjustable transcription control in mammalian cells and mice.Nucleic acids research · 2007Article
- Current status of transcriptional regulation systems.Cytotechnology · 2006Article
- A novel mammalian expression system derived from components coordinating nicotine degradation in arthrobacter nicotinovorans pAO1.Nucleic acids research · 2005Article
- Dual-regulated expression of C/EBP-alpha and BMP-2 enables differential differentiation of C2C12 cells into adipocytes and osteoblasts.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advanced heterologous transcription control systems for adjusting desired transgene expression are essential for gene function assignments, drug discovery, manufacturing of difficult to produce protein pharmaceuticals and precise dosing of gene-based therapeutic interventions. Conversion of the Streptomyces albus heat shock response regulator (RheA) into an artificial eukaryotic transcription factor resulted in a vertebrate thermosensor (CTA; cold-inducible transactivator), which is able to adjust transcription initiation from chimeric target promoters (P(CTA)) in a low-temperature- inducible manner. Evaluation of the temperature-dependent CTA-P(CTA) interaction using a tailored ELISA-like cell-free assay correlated increased affinity of CTA for P(CTA) with temperature downshift. The temperature-inducible gene regulation (TIGR) system enabled tight repression in the chicken bursal B-cell line DT40 at 41 degrees C as well as precise titration of model product proteins up to maximum expression at or below 37 degrees C. Implantation of microencapsulated DT40 cells engineered for TIGR-controlled expression of the human vascular endothelial growth factor A (hVEGF121) provided low-temperature-induced VEGF-mediated vascularization in chicken embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.