ArticleNucleic acids research2003
Nuclear matrix association of the human beta-globin locus utilizing a novel approach to quantitative real-time PCR.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Matrix association region/scaffold attachment region: the crucial player in defining the positions of chromosome breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells.BMC medical genomics · 2019Article
- Matrix association region/scaffold attachment region (MAR/SAR) sequence: its vital role in mediating chromosome breakages in nasopharyngeal epithelial cells via oxidative stress-induced apoptosis.BMC molecular biology · 2018Article
- Organization of sperm DNA by the nuclear matrix.American journal of clinical and experimental urology · 2018Review
- Myc-induced anchorage of the rDNA IGS region to nucleolar matrix modulates growth-stimulated changes in higher-order rDNA architecture.Nucleic acids research · 2014Article
- Kaposi's Sarcoma-Associated Herpesvirus Genome Replication, Partitioning, and Maintenance in Latency.Frontiers in microbiology · 2012Article
- Article
- Function of sperm chromatin structural elements in fertilization and development.Molecular human reproduction · 2010Review
- QPCR: Application for real-time PCR data management and analysis.BMC bioinformatics · 2009Article
- Silencing by nuclear matrix attachment distinguishes cell-type specificity: association with increased proliferation capacity.Nucleic acids research · 2009Article
- Differential nuclear scaffold/matrix attachment marks expressed genes.Human molecular genetics · 2009Article
- Maintenance of a functional higher order chromatin structure: The role of the nuclear matrix in normal and disease states.Gene therapy & molecular biology · 2009Article
- Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.PloS one · 2009Article
- Real-time PCR quantification using a variable reaction efficiency model.Analytical biochemistry · 2008Article
- Beta-globin LCR and intron elements cooperate and direct spatial reorganization for gene therapy.PLoS genetics · 2008Article
- Anchoring the genome.Genome biology · 2008Review
- Decondensing the protamine domain for transcription.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A comparative study of S/MAR prediction tools.BMC bioinformatics · 2007Article
- Selective matrix attachment regions in T helper cell subsets support loop conformation in the Ifng gene.Genes and immunity · 2007Article
- Tracking chromatin states using controlled DNase I treatment and real-time PCR.Cellular & molecular biology letters · 2007Article
- Genomic localization of RNA binding proteins reveals links between pre-mRNA processing and transcription.Genome research · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The human beta-globin locus is home to five genes that are regulated in a tissue-specific and developmental stage-specific manner. While the exact mode of expression remains somewhat enigmatic, a significant effort has been focused at the locus control region (LCR). The LCR is marked by five DNase I-hypersensitive sites (HS) approximately 15 kb upstream of the epsilon-globin gene. Nuclear matrix-associated regions (MARs) organize chromatin into functional domains and at least one of the HS appears bound to the nuclear matrix. We have employed an in vivo based PCR MAR assay to investigate the role of MAR-mediated regulation of the beta-globin locus. This was facilitated with a novel reaction efficiency based quantitative real-time PCR analysis software tool, Target Analysis Quantification. Using a log-linear regression strategy, discordances were eliminated. This allowed us to reliably estimate the relative amount of initial template associated with the nuclear matrix at 15 unique regions spanning the beta-globin locus in both non-expressing and expressing cell lines. A dynamic association dependent on expression status was revealed both at the LCR/5'HS region and within the second intron of the beta-globin gene. These results provide the first evidence that nuclear matrix association dynamically mediates the looping of the beta-globin locus to achieve transcriptional control.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.