ArticleNucleic acids research2003
Canonical nucleosides can be utilized by T4 DNA ligase as universal template bases at ligation junctions.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 37 citations in OpenAlex.
- One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics.Cell reports methods · 2024Article
- A New Specific and Sensitive RT-qPCR Method Based on Splinted 5' Ligation for the Quantitative Detection of RNA Species Shorter than microRNAs.Non-coding RNA · 2021Article
- Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase.Biopolymers · 2021Article
- 3' Branch ligation: a novel method to ligate non-complementary DNA to recessed or internal 3'OH ends in DNA or RNA.DNA research : an international journal for rapid publication of reports on genes and genomes · 2019Article
- Sequencing of lariat termini in S. cerevisiae reveals 5' splice sites, branch points, and novel splicing events.RNA (New York, N.Y.) · 2016Article
- A high-throughput assay for the comprehensive profiling of DNA ligase fidelity.Nucleic acids research · 2016Article
- Identification of DNA lesions using a third base pair for amplification and nanopore sequencing.Nature communications · 2015Article
- Efficient assembly of very short oligonucleotides using T4 DNA Ligase.BMC research notes · 2010Article
- Template-directed ligation of tethered mononucleotides by t4 DNA ligase for kinase ribozyme selection.PloS one · 2010Article
- Selectivity of Enzymatic Conversion of Oligonucleotide Probes during Nucleotide Polymorphism Analysis of DNA.Acta naturae · 2010Article
- Improvement of DNA adenylation using T4 DNA ligase with a template strand and a strategically mismatched acceptor strand.Bioorganic chemistry · 2008Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T4 DNA ligase catalyzes the template-dependent ligation of DNA. Using T4 DNA ligase under specific experimental conditions, we demonstrate that each of the four canonical nucleosides, centrally located on a template molecule such that they flank the site of ligation, can direct the ligation of nucleic acids regardless of the identity of the terminal nucleosides being covalently joined. This universal templating capability extends to those positions adjacent to the ligation junction. This is the first report, irrespective of the ligation method used or the identity of the template nucleosides (including analogs), which shows that nucleosides can act essentially as universal templates at ligation junctions in vitro. The canonical nucleosides do, however, differ in their ability to template sequence- independent ligations, with thymidine and guanosine being equally effective, yet more effective than adenosine and cytidine. Results indicate that hybridization strength surrounding the ligation junction is an important factor. The implications of this previously undiscovered property of T4 DNA ligase with canonical nucleosides are discussed.
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