ArticleNucleic acids research2003
Ribosomes stalling on uORF1 in the Xenopus Cx41 5' UTR inhibit downstream translation initiation.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Translational landscape during seed germination revealed by ribosome profiling.The Plant journal : for cell and molecular biology · 2026Article
- Mechanisms suppressing noncoding translation.Trends in cell biology · 2025Review
- Article
- Structural Features of 5' Untranslated Region in Translational Control of Eukaryotes.International journal of molecular sciences · 2025Review
- Translational buffering by ribosome stalling in upstream open reading frames.PLoS genetics · 2022Article
- Disrupting upstream translation in mRNAs is associated with human disease.Nature communications · 2021Article
- uORFs: ImportantInternational journal of molecular sciences · 2020Review
- Alternative ORFs and small ORFs: shedding light on the dark proteome.Nucleic acids research · 2020Review
- Impacts of uORF codon identity and position on translation regulation.Nucleic acids research · 2019Article
- Decoding sORF translation - from small proteins to gene regulation.RNA biology · 2016Review
- Species-specific exon loss in human transcriptomes.Molecular biology and evolution · 2015Article
- Translation attenuation via 3' terminal codon usage in bovine csn1s2 is responsible for the difference in αs2- and β-casein profile in milk.RNA biology · 2015Article
- Translational landscape of photomorphogenic Arabidopsis.The Plant cell · 2013Article
- Sucrose control of translation mediated by an upstream open reading frame-encoded peptide.Plant physiology · 2009Article
- Conserved upstream open reading frames in higher plants.BMC genomics · 2008Article
- Dual targeting of Arabidopsis holocarboxylase synthetase1: a small upstream open reading frame regulates translation initiation and protein targeting.Plant physiology · 2008Article
- Functional characterization of two novel 5' untranslated exons reveals a complex regulation of NOD2 protein expression.BMC genomics · 2007Article
- Translational repression of mouse mu opioid receptor expression via leaky scanning.Nucleic acids research · 2007Article
- The role of alternative translation start sites in the generation of human protein diversity.Molecular genetics and genomics : MGG · 2005Article
- Translation of the first upstream ORF in the hepatitis B virus pregenomic RNA modulates translation at the core and polymerase initiation codons.Nucleic acids research · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translation of Xenopus laevis Connexin41 mRNA is strongly controlled by the three upstream open reading frames (uORFs) in its 5' untranslated region. Mutation of uAUG1 into AAG induced a 100-fold increase in translation of a green fluorescent protein (GFP) reporter ORF. The termination codon of uORF1 was mutated and the uORF was linked in-frame with the GFP ORF, enabling visualisation of initiation at uAUG1 by synthesis of an elongated GFP form. Unexpectedly, hardly any elongated GFP was made, suggesting that translation of uORF1 in wild-type mRNA causes constraining of the entry of 40S ribosomal subunits upstream of uORF1. A rare leucine codon, the third codon of uORF1, contributed to the slow translation and thus to slow scanning. Replacement of the rare leucine codon in uORF1 with a common leucine codon stimulated GFP translation. Remarkably, the rare leucine codon, the termination codon of uORF1, uAUG2 and uAUG3 all improved recognition of uAUG1. Apparently, the block formed by a stalled ribosome on any element in uORF1 prevented the landing of new ribosomal subunits next to the cap and therefore downregulated GFP translation.
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