ArticleEukaryotic cell2003
TbMP44 is essential for RNA editing and structural integrity of the editosome in Trypanosoma brucei.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Deep mutational scanning of the RNase III-like domain inFrontiers in cellular and infection microbiology · 2024Article
- RNA editing catalytic complexes edit multiple mRNA sites non-processively in Trypanosoma brucei.Molecular and biochemical parasitology · 2023Article
- Editosome RNase III domain interactions are essential for editing and differ between life cycle stages inRNA (New York, N.Y.) · 2019Article
- The Architecture of Trypanosoma brucei editosomes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Identification by Random Mutagenesis of Functional Domains in KREPB5 That Differentially Affect RNA Editing between Life Cycle Stages of Trypanosoma brucei.Molecular and cellular biology · 2015Article
- Differential Editosome Protein Function between Life Cycle Stages of Trypanosoma brucei.The Journal of biological chemistry · 2015Article
- Polyuridylation in Eukaryotes: A 3'-End Modification Regulating RNA Life.BioMed research international · 2015Review
- Mutational analysis of Trypanosoma brucei editosome proteins KREPB4 and KREPB5 reveals domains critical for function.RNA (New York, N.Y.) · 2012Article
- KREPB6, KREPB7, and KREPB8 are important for editing endonuclease function in Trypanosoma brucei.RNA (New York, N.Y.) · 2012Article
- Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody.Nucleic acids research · 2012Article
- The trypanocidal activity of amidine compounds does not correlate with their binding affinity to Trypanosoma cruzi kinetoplast DNA.Antimicrobial agents and chemotherapy · 2011Article
- Endonuclease associations with three distinct editosomes in Trypanosoma brucei.The Journal of biological chemistry · 2011Article
- A fluorescence-based reporter substrate for monitoring RNA editing in trypanosomatid pathogens.Nucleic acids research · 2010Article
- Article
- A protein-protein interaction map of trypanosome ~20S editosomes.The Journal of biological chemistry · 2010Article
- The zinc-fingers of KREPA3 are essential for the complete editing of mitochondrial mRNAs in Trypanosoma brucei.PloS one · 2010Article
- Structure of the core editing complex (L-complex) involved in uridine insertion/deletion RNA editing in trypanosomatid mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome.RNA (New York, N.Y.) · 2009Article
- Differential functions of two editosome exoUases in Trypanosoma brucei.RNA (New York, N.Y.) · 2009Article
- The KREPA3 zinc finger motifs and OB-fold domain are essential for RNA editing and survival of Trypanosoma brucei.Molecular and cellular biology · 2008Article
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6 authors.
Funding
Abstract
RNA editing produces mature mitochondrial mRNAs in trypanosomatids by the insertion and deletion of uridylates. It is catalyzed by a multiprotein complex, the editosome. We identified TbMP44 among the components of enriched editosomes by a combination of mass spectrometry and DNA sequence database analysis. Inactivation of an ectopic TbMP44 allele in cells in which the endogenous alleles were disrupted abolished RNA editing, inhibited cell growth, and was eventually lethal to bloodstream form trypanosomes. Loss of TbMP44 mRNA was followed initially by a reduction in the editosome sedimentation coefficient and then by the absence of other editosome proteins despite the presence of the mRNA. Reactivation of TbMP44 gene expression resulted in the resumption of cell growth and the reappearance of editosomes. These data indicate that TbMP44 is a component of the editosome that is essential for editing and critical for the structural integrity of the editosome.
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