ArticleInvestigational new drugs2003
Different effects of vinblastine on the polymerization of isotypically purified tubulins from bovine brain.
Article in Investigational new drugs, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Disrupting VDAC1-tubulin interaction uncovers crosstalk between mitochondrial and microtubule functions with implication to cancer therapy.Cellular and molecular life sciences : CMLS · 2026Article
- Axon Regeneration: A Subcellular Extension in Multiple Dimensions.Cold Spring Harbor perspectives in biology · 2022Review
- Nuclear βII-Tubulin and its Possible Utility in Cancer Diagnosis, Prognosis and Treatment.Frontiers in cell and developmental biology · 2022Article
- Possible Roles of Specific Amino Acids in β-Tubulin Isotypes in the Growth and Maintenance of Neurons: Novel Insights From Cephalopod Mollusks.Frontiers in molecular neuroscience · 2022Article
- Article
- Interactions of β tubulin isotypes with glutathione in differentiated neuroblastoma cells subject to oxidative stress.Cytoskeleton (Hoboken, N.J.) · 2018Article
- An Emerging Role for Tubulin Isotypes in Modulating Cancer Biology and Chemotherapy Resistance.International journal of molecular sciences · 2017Review
- Novel mutations involving βI-, βIIA-, or βIVB-tubulin isotypes with functional resemblance to βIII-tubulin in breast cancer.Protoplasma · 2017Article
- Effect of CH-35, a novel anti-tumor colchicine analogue, on breast cancer cells overexpressing the βIII isotype of tubulin.Investigational new drugs · 2016Article
- Determination of the optimal tubulin isotype target as a method for the development of individualized cancer chemotherapy.Theoretical biology & medical modelling · 2013Article
- Article
- The beta isotypes of tubulin in neuronal differentiation.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Quantitative analysis of the effect of tubulin isotype expression on sensitivity of cancer cell lines to a set of novel colchicine derivatives.Molecular cancer · 2010Article
- Proteasome inhibitors increase tubulin polymerization and stabilization in tissue culture cells: a possible mechanism contributing to peripheral neuropathy and cellular toxicity following proteasome inhibition.Cell cycle (Georgetown, Tex.) · 2008Article
- Targeting cell division: small-molecule inhibitors of FtsZ GTPase perturb cytokinetic ring assembly and induce bacterial lethality.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
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Authors and funding
2 authors.
Funding
Abstract
Vinblastine, a highly successful antitumor drug, targets the tubulin molecule. Tubulin, the subunit protein of microtubules, consists of an alpha- and a beta-subunit, both of which consist of isotypes encoded by different genes. We have purified three isotypes of bovine brain tubulin, namely, alpha(beta)II, alpha(beta)III and alpha(beta)IV. Microtubule associated protein-2 (MAP2) and Tau-induced assembly of these isotypes were compared in the presence and absence of vinblastine. MAP2-induced assembly of unfractionated tubulin and all the isotypes except alpha(beta)II tubulin was resistant to 1 microM vinblastine. Vinblastine at low concentrations (< 10 microM) progressively inhibited the assembly of all of the isotypes but the vinblastine concentration required for inhibition of MAP2-induced microtubule assembly was minimal for alpha(beta)II. The tau-induced assembly of unfractionated tubulin and alpha(beta)III were equally sensitive to 1 microM vinblastine whereas alpha(beta)II and alpha(beta)IV were much more sensitive to vinblastine. The microtubules obtained in the presence of tau from unfractionated tubulin, alpha(beta)II and alpha(beta)IV could be easily aggregated by 20 microM vinblastine whereas such as aggregation of microtubules obtained from alpha(beta)III and tau required approximatedly 40 microM vinblastine. Our results suggest that among the tubulin isotypes, alpha(beta)II is the most sensitive to vinblastine in the presence of MAPs while alpha(beta)III is the most resistant and this intrinsic resistance of alpha(beta)III dimers persists in the polymeric form of alpha(beta)III tubulin as well. These results may be relevant to the therapeutic and toxic actions of vinblastine.
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Registered trials
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