ArticlePflugers Archiv : European journal of physiology1992
Activation of basolateral Cl- channels in the rat colonic epithelium during regulatory volume decrease.
Article in Pflugers Archiv : European journal of physiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 48 citations in OpenAlex.
- Parallel intermediate conductance KAmerican journal of physiology. Gastrointestinal and liver physiology · 2020Article
- Functional characterization of a ClC-2-like Cl(-) conductance in surface epithelial cells of rat rectal colon.The Journal of membrane biology · 2010Article
- TMEM16 proteins produce volume-regulated chloride currents that are reduced in mice lacking TMEM16A.The Journal of biological chemistry · 2009Article
- Methods for the study of ionic currents and Ca2+-signals in isolated colonic crypts.Biological procedures online · 2002Article
- The flavonol quercetin activates basolateral K(+) channels in rat distal colon epithelium.British journal of pharmacology · 2002Article
- Volume regulation following hypotonic shock in isolated crypts of mouse distal colon.The Journal of physiology · 1999Article
- Segmental heterogeneity of swelling-induced Cl- transport in rat small intestine.Pflugers Archiv : European journal of physiology · 1996Article
- Volume-activated chloride currents in pancreatic duct cells.The Journal of membrane biology · 1995Article
- Volume regulation in a toad epithelial cell line: role of coactivation of K+ and Cl- channels.The Journal of physiology · 1995Article
- Hypotonically activated chloride current in HSG cells.The Journal of membrane biology · 1994Article
- Swelling-induced anion and cation conductances in human epididymal cells.The Journal of physiology · 1994Article
- Activation of hyperpolarization and atypical osmosensitivity of a Cl- current in rat osteoblastic cells.The Journal of membrane biology · 1994Article
- The effect of short-chain fatty acids on Cl- and K+ conductance in rat colonic crypts.Pflugers Archiv : European journal of physiology · 1994Article
- Segmental differences along the crypt axis in the response of cell volume to secretagogues or hypotonic medium in the rat colon.Pflugers Archiv : European journal of physiology · 1994Article
- Hypotonicity activates a native chloride current in Xenopus oocytes.The Journal of general physiology · 1994Article
- Effect of short-chain fatty acids on cell volume and intracellular pH in rat distal colon.Pflugers Archiv : European journal of physiology · 1993Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exposure to a hypotonic medium caused an increase in the diameter of isolated crypts from the rat colon. The increase in cell volume was only transient and lasted about 7 min. Despite of the continuous presence of the hypotonic medium, cell volume decreased again. This regulatory volume decrease (RVD) was inhibited by 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), a Cl- channel blocker, and by Ba2+, a K+ channel blocker. Cell-attached patch-clamp recordings revealed that the RVD was associated with the activation of previously silent basolateral channels. These channels were identified after excision of the patch as Cl- channels (28 pS) and as K+ channels (45-60 pS). The RVD was dependent on the presence of external Ca2+. The phospholipase A2 inhibitor, quinacrine, and the lipoxygenase blocker, nordihydroguaiaretic acid, inhibited RVD, while indomethacin had no effect. In Ussing chamber experiments an exposure to hypotonic media caused an initial, transient increase in tissue conductance (Gt), followed by a prolonged decrease in short-circuit current (Isc) and the potential difference (V). The height of the electrical response was dependent on the decrease in the osmolarity in a range from 20 mosmol l-1 to 90 mosmol l-1. The increase in Gt was blocked by NPPB and Ba2+, whereas the decrease in Isc or V was inhibited by NPPB but enhanced by Ba2+.(ABSTRACT TRUNCATED AT 250 WORDS)
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