ArticleJournal of clinical microbiology2003
Detection and identification of Mycobacterium species isolates by DNA microarray.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Current progress of functional nanobiosensors for potential tuberculosis diagnosis: The novel way for TB control?Frontiers in bioengineering and biotechnology · 2022Pooled it
- Rapid Triage of Smear-Positive Tuberculosis Suspects with a Commercial DNA Microarray: A Real-World Experience from 1100 Sputum Specimens.Infection and drug resistance · 2026Article
- Biochip-Based Identification of Mycobacterial Species in Russia.International journal of molecular sciences · 2024Article
- The Intriguing Pattern of Nontuberculous Mycobacteria in Bulgaria and Description ofInternational journal of molecular sciences · 2024Article
- PCR-Based Microarray Enhances Diagnosis of Culture-Negative Biopsied Tissue in Patients with Invasive Mold Infections: Real-World Experience in a Tertiary Medical Center.Journal of fungi (Basel, Switzerland) · 2024Article
- Surgical site infections by atypical mycobacteria: prevalence and species characterization using MALDI-TOF and molecular LCD chip array.Infection · 2022Article
- Simultaneous Detection of Mycobacterium tuberculosis and Atypical Mycobacteria by DNA-Microarray in Egypt.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2022Article
- Nontuberculous mycobacteria in drinking water systems - the challenges of characterization and risk mitigation.Current opinion in biotechnology · 2019Review
- Advantages and limitations of potential methods for the analysis of bacteria in milk: a review.Journal of food science and technology · 2016Review
- Evaluation of a low-density hydrogel microarray technique for mycobacterial species identification.Journal of clinical microbiology · 2015Article
- Solid and Suspension Microarrays for Microbial Diagnostics.Methods in microbiology · 2015Article
- Demonstrating a multi-drug resistant Mycobacterium tuberculosis amplification microarray.Journal of visualized experiments : JoVE · 2014Article
- Semi-automated, occupationally safe immunofluorescence microtip sensor for rapid detection of Mycobacterium cells in sputum.PloS one · 2014Article
- atpE gene as a new useful specific molecular target to quantify Mycobacterium in environmental samples.BMC microbiology · 2013Article
- Novel primer-probe sets for detection and identification of mycobacteria by PCR-microarray assay.Journal of clinical microbiology · 2012Article
- Detection of IS6110 and HupB gene sequences of Mycobacterium tuberculosis and bovis in the aortic tissue of patients with Takayasu's arteritis.BMC infectious diseases · 2012Article
- Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.Applied and environmental microbiology · 2010Article
- Biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum.Journal of clinical microbiology · 2010Article
- Simple PCR-based DNA microarray system to identify human pathogenic fungi in skin.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid identification of Mycobacterium species isolates is necessary for the effective management of tuberculosis. Recently, analysis of DNA gyrase B subunit (gyrB) genes has been identified as a suitable means for the identification of bacterial species. We describe a microarray assay based on gyrB gene sequences that can be used for the identification of Mycobacteria species. Primers specific for a gyrB gene region common to all mycobacteria were synthesized and used for PCR amplification of DNA purified from clinical samples. A set of oligonucleotide probes for specific gyrB gene regions was developed for the identification of 14 Mycobacterium species. Each probe was spotted onto a silylated glass slide with an arrayer and used for hybridization with fluorescently labeled RNA derived from amplified sample DNA to yield a pattern of positive spots. This microarray produced unique hybridization patterns for each species of mycobacteria and could differentiate closely related bacterial species. Moreover, the results corresponded well with those obtained by the conventional culture method for the detection of mycobacteria. We conclude that a gyrB-based microarray can rapidly detect and identify closely related mycobacterial species and may be useful in the diagnosis and effective management of tuberculosis.
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