Evidence map›Paper›PMID 12789525›Full record

ArticlePlant cell reports2003

Long-term expression of the uidA gene in Gladiolus plants under control of either the ubiquitin, rolD, mannopine synthase, or cauliflower mosaic virus promoters following three seasons of dormancy.

K K Kamo

Abstract read
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In one paragraph

Article in Plant cell reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.1field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

K K KamoFloral and Nursery Plants Research Unit, B-010A, Room 238, U.S. Department of Agriculture National Arboretum, BARC West, Beltsville, MD 20705-2350, USA. kkamo@asrr.arsusda.gov
U.S. National Arboretum · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

UidA silencing did not occur following three seasons of dormancy for 23 independently transformed lines of Gladiolus plants carrying the bar- uidA fusion gene under control of either the cauliflower mosaic virus 35S (CaMV 35S), ubiquitin ( UBQ3), mannopine synthase ( mas2), or rolD promoters. The highest levels of GUS (beta-glucuronidase) expression were observed in callus, shoots, and roots of plants carrying the bar- uidA fusion gene under control of the CaMV 35S promoter and in shoots and roots of greenhouse-grown plants that contained the rolD promoter. There was no major difference in GUS expression when plants carrying the fusion gene driven by either the CaMV 35S, mas2, or UBQ3 promoters were grown in vitro as compared to growth in the greenhouse, although plants containing the rolD promoter expressed at 4- to 11-fold higher levels in shoots and roots, respectively, when grown in the greenhouse. The levels of GUS expression in greenhouse-grown plants were higher in roots than shoots for all four promoters. Of the 21 plants analyzed, 20 contained one to three copies of the bar- uidA fusion gene. Of the 23 plants analyzed, 11 had rearrangements of the transgene, but without apparent effects on levels of GUS expression.

Indexed as

Bacterial ProteinsCaulimovirusCulture TechniquesGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantGlucuronidaseHydro-LyasesIridaceaePlant RootsPlants, Genetically ModifiedPlant ShootsPromoter Regions, GeneticRecombinant Fusion ProteinsRegenerationUbiquitinBacterial ProteinsGlucuronidaseHydro-Lyasesmannopine synthaseRecombinant Fusion ProteinsUbiquitin

Identifiers

PMID12789525
OpenAlexW1554657679

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.