ArticlePlant cell reports2003
Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos.
Article in Plant cell reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- New Epigenetic Modifier Inhibitors Enhance Microspore Embryogenesis in Bread Wheat.Plants (Basel, Switzerland) · 2024Article
- Doubled Haploid Onion (Allium cepa L.) Production Via In Vitro Gynogenesis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Species with Haploid or Doubled Haploid Protocols.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Doubled haploid production from Spanish onion (Allium cepa L.) germplasm: embryogenesis induction, plant regeneration and chromosome doubling.Frontiers in plant science · 2015Article
- The relationship between induction of embryogenesis and chromosome doubling in microspore cultures.Protoplasma · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel approach for chromosome doubling that consists of treating embryos instead of parts of micropropagated plants was investigated. Following 2-year trials, amiprofos-methyl (APM) was found to be superior to oryzalin on the basis of a lower toxicity, and we were able to narrow the range of concentrations of APM. The addition of 2% dimethyl sulphoxide (DMSO) and 1% Triton X-100 to 25 microM APM had no effect in all treatments. A final experiment with 6,658 embryos demonstrated that a 2-day treatment in liquid media supplemented with 50 microM APM was the most successful with respect to chromosome doubling-36.7% of the plants were diploid-but the survival rate was reduced to 52.5% of that of the non-treated control. A 2-day treatment in liquid medium supplemented with 25 microM APM or a 2-day treatment on solid medium with 50 microM APM resulted in the production of diploids at a frequency of 28.9% and 21.3%, respectively. These may represent alternative methods for chromosome doubling since compared to the untreated control these two treatments reduced the survival rate by only about 24%. Final ploidy and fertility of the large proportion of induced mixoploid plants (up to 30.3%) need to be evaluated in further studies.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.