ArticlePlant cell reports2003
Gene silencing in transgenic soybean plants transformed via particle bombardment.
Article in Plant cell reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 74 citations in OpenAlex.
- Genetic transformation of legumes: an update.Plant cell reports · 2021Review
- Site-directed mutagenesis by biolistic transformation efficiently generates inheritable mutations in a targeted locus in soybean somatic embryos and transgene-free descendants in the TTransgenic research · 2021Article
- Convergence ofFrontiers in plant science · 2021Article
- Improved soybean transformation for efficient and high throughput transgenic production.Transgenic research · 2020Article
- Impacts of elevated COScientific reports · 2017Article
- Article
- CONTRAILS: A tool for rapid identification of transgene integration sites in complex, repetitive genomes using low-coverage paired-end sequencing.Genomics data · 2015Article
- Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate.Plant cell reports · 2015Article
- Seed coat pigmentation in transgenic soybean expressing the silencing suppressor 2b gene of Cucumber mosaic virus.Plant cell reports · 2013Article
- Enhanced transgene expression in sugarcane by co-expression of virus-encoded RNA silencing suppressors.PloS one · 2013Article
- Striga parasitizes transgenic hairy roots of Zea mays and provides a tool for studying plant-plant interactions.Plant methods · 2012Article
- RNA silencing as a tool to uncover gene function and engineer novel traits in soybean.Breeding science · 2012Article
- Recent advances in soybean transformation and their application to molecular breeding and genomic analysis.Breeding science · 2012Article
- Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds.Transgenic research · 2010Article
- Efficient and stable transformation of hop (Humulus lupulus L.) var. Eroica by particle bombardment.Plant cell reports · 2008Article
- The transcriptional activity of WT1 gene promoter and enhancer in cell lines with diverse tissue origin.International journal of hematology · 2008Article
- Expression of a synthetic cry1EC gene for resistance against Spodoptera litura in transgenic peanut (Arachis hypogaea L.).Plant cell reports · 2008Article
- Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene.Plant cell reports · 2007Article
- Expression of a feedback insensitive anthranilate synthase gene from tobacco increases free tryptophan in soybean plants.Plant cell reports · 2007Article
- Development of resistant transgenic soybeans with inverted repeat-coat protein genes of soybean dwarf virus.Plant cell reports · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenes are susceptible to silencing in plants especially when multiple copies of the gene of interest are introduced. Transgenic plants derived by particle bombardment, which is the common method for transforming soybean, have a tendency to have multiple integration events. Three independent transgenic soybean plants obtained via particle bombardment were analyzed for transgene silencing. A GUS transgenic soybean line had at least 100 copies of the GUS gene while there were approximately 60 copies of the transgene in the two soybean lines transformed with a 15-kDa zein storage protein gene from maize. Soybean plants transformed with the GUS gene showed variable GUS expression. The coding region and promoter of the GUS gene in the plants with low expression of GUS were heavily methylated. Variability in GUS expression was observed in the progeny of the high expressors in the T(2) and T(3) generations as well. Expression level of the 15-kDa zein gene in transgenic soybean plants showed correlation with the level of transgene methylation. The helper component-proteinase from potyviruses is known to suppress post-transcriptional gene silencing. Transgenic plants were inoculated with the soybean mosaic potyvirus (SMV) to test possible effects on transgene silencing in soybean. Infection with SMV did not suppress transgene silencing in these plants and suggests that the silencing in these plants may not be due to post-transcriptional gene silencing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.