ArticleApplied and environmental microbiology2003
Consensus-degenerate hybrid oligonucleotide primers for amplification of priming glycosyltransferase genes of the exopolysaccharide locus in strains of the Lactobacillus casei group.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 50 citations in OpenAlex.
- Screening of Functional Properties of Lactic Acid Bacteria Isolated from Animal Rennets and Their Associated Cheeses and Whey.Foods (Basel, Switzerland) · 2026Article
- The Integrated Probiotic Database: a genomic compendium of bifidobacterial health-promoting strains.Microbiome research reports · 2022Article
- Exopolysaccharide production in fermented milk usingAIMS microbiology · 2022Article
- Enhancement of exopolysaccharides production and reactive oxygen species level of Nostoc flagelliforme in response to dehydration.Environmental science and pollution research international · 2021Article
- Exopolysaccharide, Isolated From a Novel StrainFrontiers in microbiology · 2019Article
- Characterization ofFrontiers in microbiology · 2018Article
- A network-based approach to identify substrate classes of bacterial glycosyltransferases.BMC genomics · 2014Article
- Genomic overview and biological functions of exopolysaccharide biosynthesis in Bifidobacterium spp.Applied and environmental microbiology · 2014Review
- Polyphasic screening, homopolysaccharide composition, and viscoelastic behavior of wheat Sourdough from a Leuconostoc lactis and Lactobacillus curvatus exopolysaccharide-producing starter culture.Applied and environmental microbiology · 2012Article
- A degenerate primer MOB typing (DPMT) method to classify gamma-proteobacterial plasmids in clinical and environmental settings.PloS one · 2012Article
- Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG.Applied and environmental microbiology · 2007Article
- Screening of exopolysaccharide-producing Lactobacillus and Bifidobacterium strains isolated from the human intestinal microbiota.Applied and environmental microbiology · 2007Article
- Diversity of heteropolysaccharide-producing lactic acid bacterium strains and their biopolymers.Applied and environmental microbiology · 2006Article
- Functional and topological analysis of the Burkholderia cenocepacia priming glucosyltransferase BceB, involved in the biosynthesis of the cepacian exopolysaccharide.Journal of bacteriology · 2005Article
- Characterization of a mobile clpL gene from Lactobacillus rhamnosus.Applied and environmental microbiology · 2005Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A primer design strategy named CODEHOP (consensus-degenerate hybrid oligonucleotide primer) for amplification of distantly related sequences was used to detect the priming glycosyltransferase (GT) gene in strains of the Lactobacillus casei group. Each hybrid primer consisted of a short 3' degenerate core based on four highly conserved amino acids and a longer 5' consensus clamp region based on six sequences of the priming GT gene products from exopolysaccharide (EPS)-producing bacteria. The hybrid primers were used to detect the priming GT gene of 44 commercial isolates and reference strains of Lactobacillus rhamnosus, L. casei, Lactobacillus zeae, and Streptococcus thermophilus. The priming GT gene was detected in the genome of both non-EPS-producing (EPS(-)) and EPS-producing (EPS(+)) strains of L. rhamnosus. The sequences of the cloned PCR products were similar to those of the priming GT gene of various gram-negative and gram-positive EPS(+) bacteria. Specific primers designed from the L. rhamnosus RW-9595M GT gene were used to sequence the end of the priming GT gene in selected EPS(+) strains of L. rhamnosus. Phylogenetic analysis revealed that Lactobacillus spp. form a distinctive group apart from other lactic acid bacteria for which GT genes have been characterized to date. Moreover, the sequences show a divergence existing among strains of L. rhamnosus with respect to the terminal region of the priming GT gene. Thus, the PCR approach with consensus-degenerate hybrid primers designed with CODEHOP is a practical approach for the detection of similar genes containing conserved motifs in different bacterial genomes.
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