ArticleApplied and environmental microbiology2003
Unusual organization for lactose and galactose gene clusters in Lactobacillus helveticus.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
- A microbiome catalog of Chinese traditional artisanal cheeses provides insights into functional and microbial diversity.Nature communications · 2026Article
- Functional characterization and immunomodulatory properties of Lactobacillus helveticus strains isolated from Italian hard cheeses.PloS one · 2021Article
- Characterization of two extracellular arabinanases in Lactobacillus crispatus.Applied microbiology and biotechnology · 2020Article
- Structural Comparison of Different Galacto-oligosaccharide Mixtures Formed by β-Galactosidases from Lactic Acid Bacteria and Bifidobacteria.Journal of agricultural and food chemistry · 2020Article
- GlaR (YugA)-a novel RpiR-family transcription activator of the Leloir pathway of galactose utilization in Lactococcus lactis IL1403.MicrobiologyOpen · 2019Article
- β-Galactosidase fromInternational journal of molecular sciences · 2019Article
- A constitutive unregulated expression of β-galactosidase in Lactobacillus fermentum M1.Current microbiology · 2015Article
- LacR is a repressor of lacABCD and LacT is an activator of lacTFEG, constituting the lac gene cluster in Streptococcus pneumoniae.Applied and environmental microbiology · 2014Article
- Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Comparative high-density microarray analysis of gene expression during growth of Lactobacillus helveticus in milk versus rich culture medium.Applied and environmental microbiology · 2007Article
- Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Genetic diversity in the lactose operons of Lactobacillus helveticus strains and its relationship to the role of these strains as commercial starter cultures.Applied and environmental microbiology · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleotide sequences of the Lactobacillus helveticus lactose utilization genes were determined, and these genes were located and oriented relative to one another. The lacLM genes (encoding the beta-galactosidase protein) were in a divergent orientation compared to lacR (regulatory gene) and lacS (lactose transporter). Downstream from lacM was an open reading frame (galE) encoding a UDP-galactose 4 epimerase, and the open reading frame had the same orientation as lacM. The lacR gene was separated from the downstream lacS gene by 2.0 kb of DNA containing several open reading frames that were derived from fragmentation of another permease gene (lacS'). Northern blot analysis revealed that lacL, lacM, and galE made up an operon that was transcribed in the presence of lactose from an upstream lacL promoter. The inducible genes lacL and lacM were regulated at the transcriptional level by the LacR repressor. In the presence of glucose and galactose galE was transcribed from its promoter, suggesting that the corresponding enzyme can be expressed constitutively. Lactose transport was inducible by addition of lactose to the growth medium.
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