ArticleBMC genetics2003
Involvement of an SCFSlmb complex in timely elimination of E2F upon initiation of DNA replication in Drosophila.
Article in BMC genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Embedded CRISPRi Enhances Gene-Silencing Efficiency in Drosophila.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Engineered degradation of EYFP-tagged CENH3 via the 26S proteasome pathway in plants.PloS one · 2021Article
- Temporal remodeling of the cell cycle accompanies differentiation in the Drosophila germline.Developmental biology · 2017Article
- MEK inhibitor effective against proliferation in breast cancer cell.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Skp2 suppresses apoptosis in Rb1-deficient tumours by limiting E2F1 activity.Nature communications · 2014Article
- Dysregulation of core components of SCF complex in poly-glutamine disorders.Cell death & disease · 2012Article
- S phase-coupled E2f1 destruction ensures homeostasis in proliferating tissues.PLoS genetics · 2012Article
- Article
- Pharmacological and genetic reversal of age-dependent cognitive deficits attributable to decreased presenilin function.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Age-dependent cognitive impairment in a Drosophila fragile X model and its pharmacological rescue.Biogerontology · 2010Article
- Intrinsic negative cell cycle regulation provided by PIP box- and Cul4Cdt2-mediated destruction of E2f1 during S phase.Developmental cell · 2008Article
- The anaphase-promoting complex/cyclosome (APC/C) is required for rereplication control in endoreplication cycles.Genes & development · 2008Article
- Article
- dDP is needed for normal cell proliferation.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCul1 is a core component of the evolutionarily conserved SCF-type ubiquitin ligases that target specific proteins for destruction. SCF action contributes to cell cycle progression but few of the key targets of its action have been identified.
resultsWe found that expression of the mouse Cul1 (mCul1) in the larval wing disc has a dominant negative effect. It reduces, but does not eliminate, the function of SCF complexes, promotes accumulation of Cubitus interruptus (a target of SCF action), triggers apoptosis, and causes a small wing phenotype. A screen for mutations that dominantly modify this phenotype showed effective suppression upon reduction of E2F function, suggesting that compromised downregulation of E2F contributes to the phenotype. Partial inactivation of Cul1 delayed the abrupt loss of E2F immunofluorescence beyond its normal point of downregulation at the onset of S phase. Additional screens showed that mild reduction in function of the F-box encoding gene slimb enhanced the mCul1 overexpression phenotype. Cell cycle modulation of E2F levels is virtually absent in slimb mutant cells in which slimb function is severely reduced. This implicates Slimb, a known targeting subunit of SCF, in E2F downregulation. In addition, Slimb and E2F interacted in vitro in a phosphorylation-dependent manner.
conclusionWe have used genetic and physical interactions to identify the G1/S transcription factor E2F as an SCFSlmb target in Drosophila. These results argue that the SCFSlmb ubiquitin ligase directs E2F destruction in S phase.
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