ArticleGenes & development2003
Transcription-dependent recombination and the role of fork collision in yeast rDNA.
Article in Genes & development, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 148 papers.
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Who cites it
148 citing papers in PubMed, 281 citations in OpenAlex.
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- Plasticity and stringency: rethinking stem cell division modes.Biochemical Society transactions · 2026Review
- Compensatory evolution to DNA replication stress is robust to nutrient availability.Molecular systems biology · 2025Article
- RNA polymerase II is a polar roadblock to a progressing DNA fork.Nature communications · 2025Article
- Participants in Transcription-Replication Conflict and Their Role in Formation and Resolution of R-Loops.International journal of molecular sciences · 2025Review
- Article
- Insulin signaling regulates R2 retrotransposon expression to orchestrate transgenerational rDNA copy number maintenance.Nature communications · 2025Article
- A mortality timer based on nucleolar size triggers nucleolar integrity loss and catastrophic genomic instability.Nature aging · 2024Article
- Review
- Replication fork blocking deficiency leads to a reduction of rDNA copy number in budding yeast.iScience · 2024Article
- Acidic growth conditions stabilize the ribosomal RNA gene cluster and extend lifespan through noncoding transcription repression.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- RNA polymerase I mutant affects ribosomal RNA processing and ribosomal DNA stability.RNA biology · 2024Article
- Review
- The retrotransposon R2 maintainsProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Strand asymmetries across genomic processes.Computational and structural biotechnology journal · 2023Review
- Structural insights into nuclear transcription by eukaryotic DNA-dependent RNA polymerases.Nature reviews. Molecular cell biology · 2022Review
- Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling.Nature cell biology · 2022Article
- Article
- The human ribosomal DNA array is composed of highly homogenized tandem clusters.Genome research · 2021Article
- Smc5/6 in the rDNA modulates lifespan independently of Fob1.Aging cell · 2021Article
88 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is speculated that the function of the replication fork barrier (RFB) site is to avoid collision between the 35S rDNA transcription machinery and the DNA replication fork, because the RFB site is located near the 3'-end of the gene and inhibits progression of the replication fork moving in the opposite direction to the transcription machinery. However, the collision has never been observed in a blockless (fob1) mutant with 150 copies of rDNA. The gene FOB1 was shown previously to be required for replication fork blocking activity at the RFB site, and also for the rDNA copy number variation through unequal sister-chromatid recombination. This study documents the detection of fork collision in an fob1 derivative with reduced rDNA copy number (approximately 20) using two-dimensional agarose gel electrophoresis. This suggests that most of these reduced copies are actively transcribed. The collision was dependent on the transcription by RNA polymerase I. In addition, the transcription stimulated rDNA copy number variation, and the production of the extrachromosomal rDNA circles (ERCs), whose accumulation is thought to be a cause of aging. These results suggest that such a transcription-dependent fork collision induces recombination, and may function as a general recombination trigger for multiplication of highly transcribed single-copy genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.