ArticleThe Journal of experimental medicine2003
Marrow stem cells shift gene expression and engraftment phenotype with cell cycle transit.
Article in The Journal of experimental medicine, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 81 citations in OpenAlex.
- Safety run-in and part 1 of GIMEMA AML1718: venetoclax combined with FLAI as induction treatment in non-low-risk AML.Blood advances · 2025Trial
- The Essence of Quiescence.Stem cells and development · 2024Review
- The universal stem cell.Leukemia · 2022Review
- Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage.Stem cell reviews and reports · 2022Article
- Concise reviews: A stem cell apostasy: a tale of four H words.Stem cells (Dayton, Ohio) · 2015Review
- Reconsidering marrow stem cell cycle status: insights into an actively cycling hematopoietic stem cell population.Leukemia supplements · 2014Article
- Article
- DNA damage enhanced by the attenuation of SLD5 delays cell cycle restoration in normal cells but not in cancer cells.PloS one · 2014Article
- Progenitor/stem cell fate determination: interactive dynamics of cell cycle and microvesicles.Stem cells and development · 2012Article
- Homing and long-term engraftment of long- and short-term renewal hematopoietic stem cells.PloS one · 2012Article
- Recruitment and activation of pancreatic stellate cells from the bone marrow in pancreatic cancer: a model of tumor-host interaction.PloS one · 2011Article
- Expression of cell cycle-related genes with cytokine-induced cell cycle progression of primitive hematopoietic stem cells.Stem cells and development · 2010Article
- Heterogeneity of non-cycling and cycling synchronized murine hematopoietic stem/progenitor cells.Journal of cellular physiology · 2010Article
- The paradoxical dynamism of marrow stem cells: considerations of stem cells, niches, and microvesicles.Stem cell reviews · 2008Review
- Gene expression fluctuations in murine hematopoietic stem cells with cell cycle progression.Journal of cellular physiology · 2008Article
- Leukemia and hematopoietic stem cells: balancing proliferation and quiescence.Cell cycle (Georgetown, Tex.) · 2008Review
- Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing.Experimental cell research · 2007Article
- Murine allogeneic in vivo stem cell homing(,).Journal of cellular physiology · 2007Article
- SHIP deficiency enhances HSC proliferation and survival but compromises homing and repopulation.Blood · 2006Article
- Global gene expression profiling of cells overexpressing SMC3.Molecular cancer · 2005Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
We studied the genetic and engraftment phenotype of highly purified murine hematopoietic stem cells (lineage negative, rhodamine-low, Hoechst-low) through cytokine-stimulated cell cycle. Cells were cultured in interleukin (IL)-3, IL-6, IL-11, and steel factor for 0 to 48 h and tested for engraftment capacity in a lethally irradiated murine competitive transplant model. Engraftment showed major fluctuations with nadirs at 36 and 48 h of culture and recovery during the next G1. Gene expression of quiescent (0 h) or cycling (48 h) stem cells was compared with lineage positive cells by 3' end PCR differential display analysis. Individual PCR bands were quantified using a 0 to 9 scale and results were visually compared using color-coded matrices. We defined a set of 637 transcripts expressed in stem cells and not expressed in lineage positive cells. Gene expression analyzed at 0 and 48 h showed a major shift from "stem cell genes" being highly expressed at 0 h and turned off at 48 h, while "cell division" genes were turned on at 48 h. These observations suggest stem cell gene expression shifts through cell cycle in relation to cell cycle related alterations of stem cell phenotype. The engraftment defect is related to a major phenotypic change of the stem cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.